Multiplex PCR amplification using Extractor probes
(English)Manuscript (preprint) (Other academic)
Manipulation of a plurality of DNA molecules in parallel offers several advantages over simplex reactions. This paper reports on a new version of Selector probes with easier design procedure and the possibility to achieve higher ROI content within the targeted fragments. To demonstrate its utility we applied it for copy number variance detection and simultaneous amplification of 64 genomic fragments.
PCR, sequencing, genetics, CNV
Research subject Genetics
IdentifiersURN: urn:nbn:se:uu:diva-121532OAI: oai:DiVA.org:uu-121532DiVA: diva2:305662