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Enhancement of antibody responses by DNA immunization using expression vectors mediating efficient antigen secretion
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. (Lobell / Kämpe)
1999 (English)In: JIM - Journal of Immunological Methods, ISSN 0022-1759, E-ISSN 1872-7905, Vol. 228, no 1-2, 121-130 p.Article in journal (Refereed) Published
Abstract [en]

The immune responses elicited in mice, after intradermal (i.d.) immunization with plasmids encoding secreted or intracellular forms of HIV-1 nef, HIV-1 tat or C. pneumoniae omp2 proteins, respectively, were compared. To mediate secretion of these proteins the genes were fused to a heterologous signal sequence from murine heavy chain IgG. The nef- and omp2-specific antibody responses were dramatically increased when mice were inoculated with the plasmid encoding the secreted form of these proteins. In contrast, HIV-1 tat comprising an internal strong nuclear targeting sequence could not be induced to secretion and subsequently no enhanced antibody response was observed. Slight improvement of the HIV-1 nef antibody response was achieved after co-inoculation with a granulocyte-macrophage colony-stimulating factor (GM-CSF) expression vector. Further, nef-specific T-cell responses were induced after nef DNA injections, and were of Th1-like phenotype regardless of whether the nef protein was secreted or not. The system described in this study, using a plasmid vector with a strong heterologous signal sequence that mediate efficient antigen secretion in vivo, may have wide applicability for the induction of high antibody levels to normally non-secreted antigens.

Place, publisher, year, edition, pages
1999. Vol. 228, no 1-2, 121-130 p.
Keyword [en]
DNA immunization, Signal sequence, nef, omp2, Antibody response
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-121540DOI: 10.1016/S0022-1759(99)00086-1PubMedID: 10556549OAI: oai:DiVA.org:uu-121540DiVA: diva2:305676
Available from: 2010-03-24 Created: 2010-03-24 Last updated: 2010-05-25Bibliographically approved

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