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FemtoMolar measurements using accelerator mass spectrometry
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics. (jonfysik)
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics. (jonfysik)
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Ion Physics. (jonfysik)
2009 (English)In: Rapid Communications in Mass Spectrometry, ISSN 0951-4198, E-ISSN 1097-0231, Vol. 23, no 5, 557-563 p.Article in journal (Refereed) Published
Abstract [en]

Accelerator mass spectrometry (AMS) is an ultra-sensitive analytical  method suitable for the detection of sub-nM concentrations of labeled   biological substances such as pharmaceutical drugs in body fluids. A  limiting factor in extending the concentration measurements to the   sub-pM range is the natural C-14 content in living tissues. This was  circumvented by separating the labeled drug from the tissue matrix,  using standard high-performance liquid chromatography (HPLC)   procedures. As the separated total drug amount is in the few fg range,  it is not possible to use a standard AMS sample preparation method,  where mg sizes are required. We have utilized a sensitive carbon carrier method where a C-14-deficient compound is added to the HPLC fractions and the composite sample is prepared and analyzed by AMS.   Using 50 mu L human blood plasma aliquots, we have demonstrated concentration measurements below 20 JFM, containing sub-amol amounts of   the labeled drug. The method has the immediate potential of operating in the sub-fM region.

Place, publisher, year, edition, pages
2009. Vol. 23, no 5, 557-563 p.
National Category
Subatomic Physics Engineering and Technology
Research subject
Ion Physics
Identifiers
URN: urn:nbn:se:uu:diva-122068DOI: 10.1002/rcm.3903ISI: 000263795000002OAI: oai:DiVA.org:uu-122068DiVA: diva2:308375
Available from: 2010-04-06 Created: 2010-04-06 Last updated: 2017-12-12Bibliographically approved

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Salehpour, MehranPossnert, Göran

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