FemtoMolar measurements using accelerator mass spectrometry
2009 (English)In: Rapid Communications in Mass Spectrometry, ISSN 0951-4198, E-ISSN 1097-0231, Vol. 23, no 5, 557-563 p.Article in journal (Refereed) Published
Accelerator mass spectrometry (AMS) is an ultra-sensitive analytical method suitable for the detection of sub-nM concentrations of labeled biological substances such as pharmaceutical drugs in body fluids. A limiting factor in extending the concentration measurements to the sub-pM range is the natural C-14 content in living tissues. This was circumvented by separating the labeled drug from the tissue matrix, using standard high-performance liquid chromatography (HPLC) procedures. As the separated total drug amount is in the few fg range, it is not possible to use a standard AMS sample preparation method, where mg sizes are required. We have utilized a sensitive carbon carrier method where a C-14-deficient compound is added to the HPLC fractions and the composite sample is prepared and analyzed by AMS. Using 50 mu L human blood plasma aliquots, we have demonstrated concentration measurements below 20 JFM, containing sub-amol amounts of the labeled drug. The method has the immediate potential of operating in the sub-fM region.
Place, publisher, year, edition, pages
2009. Vol. 23, no 5, 557-563 p.
Subatomic Physics Engineering and Technology
Research subject Ion Physics
IdentifiersURN: urn:nbn:se:uu:diva-122068DOI: 10.1002/rcm.3903ISI: 000263795000002OAI: oai:DiVA.org:uu-122068DiVA: diva2:308375