The quest for molecular quasi-species in ligand-activity space and its application to directed enzyme evolution
2010 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 584, no 12, 2565-2571 p.Article, review/survey (Refereed) Published
We propose that the proper evolving unit in enzyme evolution is not a single "fittestmolecule", but a cluster of related variants denoted a "quasi-species". A distribution of variantsprovides genetic variability and thereby reduces the risk of inbreeding and evolutionary dead-ends.Different matrices of substrates or activity modulators will lead to different selection criteria anddivergent evolutionary trajectories. We provide examples from our directed evolution of glutathionetransferases illustrating the interplay between libraries of enzyme variants and ligand matrices in theidentification of quasi-species. The ligand matrix is shown to be crucial to the outcome of the search fornovel activities. In this sense the experimental system resembles the biological environment ingoverning the evolution of enzymes.
Place, publisher, year, edition, pages
2010. Vol. 584, no 12, 2565-2571 p.
quasi-species, glutathione transferase, enzyme evolution, ligand space, activity space, multivariate analysis
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-122376DOI: 10.1016/j.febslet.2010.04.024ISI: 000278031800010OAI: oai:DiVA.org:uu-122376DiVA: diva2:309930