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Fast multi-element screening of non-digested biological materials by slurry introduction to ICP-AES.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry.
2010 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 80, no 5, 2068-2075 p.Article in journal (Refereed) Published
Abstract [en]

A fast method for direct multi-element analysis of non-digested biological samples is presented. The only sample preparation needed is 1 min homogenization with a Polytron mixer in a small volume of neutral phosphate buffer saline solution (PBS). The total time for analysis (sample preparation and measurement) is 4 min only. This "mix and measure" method can handle large sample loads of biological samples and thus minimize dilution of trace elements. For example 100% whole blood was introduced without any clogging of the introduction system or extinguishing of the plasma. In 70% (v/v) whole blood reference material 14 of 16 analytes were quantified within ±10% (Al, B, Ba, Ca, Cu, Fe, Mg, Mn, P, Pb, S, Sr, Ti and Zn) and two semi-quantified within ±20% (Cd and K). Fresh bovine liver was also analyzed with the same method and 7 of 9 analytes were quantified in 5% (w/v) liver slurry. Three different nebulizers were tested, Glass Expansion Concentric (GEC) of Meinhard type, Cross Flow and Burgener T2100 and they performed roughly equally well in giving quantitative results for the slurries but the sensitivity was better with the GEC. The stability of the plasma was studied by evaluating the ratio of Mg 280.270 nm and Mg 285.213 nm lines. When increasing the sample load from 20 to 100% (v/v) of whole blood and from 0.5 to 10% (w/v) of bovine liver the Mg ratio was constant within a few percent for all of the nebulizer tested. The ratio of the sensitivity between GEC and Burgener T2100 was studied and the ratio increased with the energy sum for atomic and ionic lines separately.

Place, publisher, year, edition, pages
2010. Vol. 80, no 5, 2068-2075 p.
Keyword [en]
External calibration, ICP-AES, internal standards, liver, slurry, whole blood
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-122404DOI: 10.1016/j.talanta.2009.11.007ISI: 000275612500073PubMedID: 20152454OAI: oai:DiVA.org:uu-122404DiVA: diva2:310023
Available from: 2010-04-12 Created: 2010-04-12 Last updated: 2017-12-12Bibliographically approved
In thesis
1. Inductively Coupled Plasma Atomic Emission Spectrometry: Exploring the Limits of Different Sample Preparation Strategies
Open this publication in new window or tab >>Inductively Coupled Plasma Atomic Emission Spectrometry: Exploring the Limits of Different Sample Preparation Strategies
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis describes two different sample preparation strategies for inductively coupled plasma atomic emission spectrometry (ICP-AES), and their ability regarding multi element quantification in complex samples. Sensitivity, repeatability, reproducibility and accuracy were investigated. The aim was to increase the over all efficiency, the speed of analysis, and/or the sensitivity of the analytical method. The intention was to measure analytes with concentrations ranging from ng/g to mg/g simultaneously. The aim was additionally to study chemical and physical processes occurring during the sample preparation, the sample transport to the plasma, and the atomization therein.

In the first sample preparation strategy, a hydrophilic highly cross-linked iminodiacetate-agarose adsorbent, IDA-Novarose, was used for preconcentration of metal ions, and matrix elimination in natural water samples. The sorbent was synthesized with different binding capacities. The effect of the capacity on preconcentration, matrix elimination, and uptake capability at high flow rates was studied. For a high capacity IDA-Novarose (≥ 45 µmole/ml) quantitative uptake was seen even at high flow rates (100 ml/min) for Cu2+ with a high affinity to the adsorbent, and for Cd2+ with a moderate affinity. For lower capacities the uptake of Cd2+ was affected by the sample matrix and the flow rate. A method based on the determination of the conditional stability constant of the metal sorbent complex was suggested for the prediction of the sorbent capacity needed to obtain quantitative recovery and optimal matrix elimination. The sorbent was used in a flow system with online buffering for the analysis of a certified riverine water (SLRS-3), tap water and lake water. With few exceptions the results obtained by ICP-AES after preconcentration agreed well with the certified concentrations and results obtained by ICP-MS.

The other sample preparation strategy discussed is a method for non digested biological samples from different animal organs for the multi element analysis by ICP-AES. This “mix and measure method” consists of a simple homogenization of the sample with a mixing rod in a small amount of neutral media, followed by dilution and direct measurement with ICP-AES. The total time of analysis is only a few minutes. The ability of this fast method to accurately quantify some elements of toxic, environmental, and/or physiological concern with the lowest possible sample dilution and the highest possible plasma load was evaluated. In 10 % liver slurry Cd, Co, and Sr, at concentration levels around 0.05 µg/g were quantified simultaneously with P and K around 2000 µg/g and with several other elements in between (Al, Ca, Cu, Fe, Mg, Mn, Pb, and Zn). The relative standard deviation of repeated measurements of samples was around 5 - 6 % for regardless of the concentration of the element. The method was also used for fast screening of the elemental distribution in mice organs (brain, heart, kidney, liver, lung and spleen).

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2011. 59 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 821
Keyword
multi element analysis, trace element, ICP-AES, inductively coupled plasma atomic emission spectromtery, water analysis, liver analysis, non digested samples, medical samples, biological samples, samples from biopsies and autopsies, mice organ, fast analysis, internal standard, preconcentration, matrix elimination, Cu, Zn, Mn, Co, Pb, Cd, Mg, Ca, Fe, Ni, Cr, S, P, K, Al
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-150861 (URN)978-91-554-8063-9 (ISBN)
Public defence
2011-05-20, B22, Uppsala Biomedical Center, BMC, Husargatan 3, Uppsala, 10:15 (English)
Opponent
Supervisors
Available from: 2011-04-29 Created: 2011-04-06 Last updated: 2011-05-05Bibliographically approved

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