Application of artificial gel antibodies for investigating molecular polymorphisms of human pituitary growth hormone
2011 (English)In: Amino Acids, ISSN 0939-4451, E-ISSN 1438-2199, Vol. 40, no 4, 1249-1255 p.Article in journal (Refereed) Published
Artificial gel-antibodies were used to probe human growth hormone (GH) activity in hormone preparations purified from human pituitaries. A partially purified fraction containing differently sized forms of the hormone was further processed to yield monomeric and dimeric forms of GH activity suitable for synthesizing artificial polyacrylamide gel antibodies. These two types of GH antibodies were used for probing GH activity in experiments analyzing the two forms of the hormone by HPLC gel-permeation and ion-exchange chromatography. In the size-exclusion experiments both hormone fractions eluted as homogenous peaks, whereas the ion exchanger resolved the hormones in several active components. The antibodies towards the GH monomer were more potent to recognize monomeric GH compared to antibodies against dimeric GH. The opposite was found for the dimeric GH antibodies. It was concluded that these two sets of antibodies it might be useful for discriminating between dimeric and monomeric GH in samples of clinical origin.
Place, publisher, year, edition, pages
2011. Vol. 40, no 4, 1249-1255 p.
Assay, Electrophoresis, Growth hormone (GH), HPLC, Human, Pituitary, Polyacrylamide gel antibodies, Purification, Quantification
Research subject Pharmaceutical Biochemistry
IdentifiersURN: urn:nbn:se:uu:diva-122448DOI: 10.1007/s00726-011-0840-3ISI: 000288546700021PubMedID: 21312046OAI: oai:DiVA.org:uu-122448DiVA: diva2:310166