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Nuclear expression of the RNA-binding protein RBM3 is associated with an improved clinical outcome in breast cancer
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
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2009 (English)In: Modern Pathology, ISSN 0893-3952, E-ISSN 1530-0285, Vol. 22, no 12, 1564-1574 p.Article in journal (Refereed) Published
Abstract [en]

Single-strand RNA-binding proteins (RBPs) are involved in many aspects of RNA metabolism and in the regulation of gene transcription. The RBP RBM3 was recently suggested to be a proto-oncogene in colorectal cancer; however, such a role has not been corroborated by previous studies in the colon or other tumor types, and the prognostic implications of tumor-specific RBM3 expression remain unclear. Mono-specific antibodies against RBM3 were generated. Antibody specificity was confirmed using siRNA gene silencing, western blotting and immunohistochemistry on a panel of breast cancer cell lines. Using tissue microarrays and IHC, RBM3 protein expression was examined in 48 normal tissues and in 20 common cancers. Additional analysis in two independent breast cancer cohorts (n=1016) with long-term follow-up was also carried out. RBM3 was upregulated in cancer compared to normal tissues. The nuclear expression of RBM3 in breast cancer was associated with low grade (P<0.001), small tumors (P<0.001), estrogen receptor (ER) positivity (P<0.001) and Ki-67 negativity (P<0.001) in both the breast cancer cohorts. An increased nuclear expression of RBM3 was associated with a prolonged overall and recurrence-free survival. The prognostic value was particularly pronounced in hormone receptor-positive tumors and remained significant in multivariate interaction analysis after controlling for tamoxifen treatment (HR: 0.49, 95% CI: 0.30-0.79, P=0.004). These data strongly indicate that nuclear RBM3 is an independent favorable prognostic factor in breast cancer, and seems to have a specific role in ER-positive tumors.

Place, publisher, year, edition, pages
2009. Vol. 22, no 12, 1564-1574 p.
Keyword [en]
RBM3, breast cancer, prognosis, estrogen receptor, antibody-based proteomics
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-122495DOI: 10.1038/modpathol.2009.124ISI: 000272207200006PubMedID: 19734850OAI: oai:DiVA.org:uu-122495DiVA: diva2:310260
Available from: 2010-04-13 Created: 2010-04-13 Last updated: 2010-06-23Bibliographically approved

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