uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Blood interactions with noble metals: coagulation and immune complement activation
Department of Cell and Molecular Biology/Interface Biophysics, Gothenburg University.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Oncology, Radiology and Clinical Immunology, Clinical Immunology.
Department of Cell and Molecular Biology/Interface Biophysics, Gothenburg University.
Department of Cell and Molecular Biology/Interface Biophysics.
Show others and affiliations
2009 (English)In: ACS Applied Materials & Interfaces, ISSN 1944-8244, Vol. 1, no 5, 1053-1062 p.Article in journal (Refereed) Published
Abstract [en]

Noble metals are interesting biomaterials for a number of reasons, e.g., their chemical inertness and relative mechanical softness, silver's long known antimicrobial properties, and the low allergenic response shown by gold. Although important for the final outcome of biomaterials, little is reported about early events between pure noble metals and blood. In this article, we used whole blood in the "slide chamber model" to study the activation of the immune complement activation, generation of thrombin/antithrombin (TAT) complexes, and platelet depletion from blood upon contact with silver (Ag), palladium (Pd), gold (Au), titanium (Ti), and Bactiguard, a commercial nanostructured biomaterial coating comprised of Ag, Pd, and Au. The results show the highest TAT generation and platelet depletion on Ti and Au and lower on Pd, Ag, and the Bactiguard coating. The immune complement factor 3 fragment (C3a) was generated by the surfaces in the following order: Ag > Au > Pd > Bactiguard > Ti. Quartz crystal microbalance adsorption studies with human fibrinogen displayed the highest deposition to Ag and the lowest onto the Bactiguard coating. The adsorbed amounts of fibrinogen did not correlate with thrombogenicity in terms of TAT formation and platelet surface accumulation in blood. The combined results suggest, hence, that noble metal chemistry has a different impact on the protein adsorption properties and general blood compatibility. The low thrombogenic response by the Bactiguard coating cannot be explained by any of the single noble metal properties but is likely a successful combination of the nanostructure, nanogalvanic effects, or combinatory chemical and physical materials properties.

Place, publisher, year, edition, pages
2009. Vol. 1, no 5, 1053-1062 p.
Keyword [en]
silver, palladium, gold, titanium, immune complement, protein adsorption, coagulation, platelets, nanostructure, QCM-D
National Category
Engineering and Technology
URN: urn:nbn:se:uu:diva-124252DOI: 10.1021/am900028eISI: 000268665200012PubMedID: 20355891OAI: oai:DiVA.org:uu-124252DiVA: diva2:317282
Available from: 2010-05-03 Created: 2010-05-03 Last updated: 2010-07-12Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
By organisation
Clinical Immunology
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 159 hits
ReferencesLink to record
Permanent link

Direct link