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Histidine-rich glycoprotein exerts antibacterial activity
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
2007 (English)In: The FEBS Journal, ISSN 1742-464X, E-ISSN 1742-4658, Vol. 274, no 2, 377-389 p.Article in journal (Refereed) Published
Abstract [en]

Histidine-rich glycoprotein (HRGP), an abundant heparin-binding protein found in plasma and thrombocytes, exerts antibacterial effects against Gram-positive bacteria (Enterococcus faecalis and Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Fluorescence studies and electron microscopy to assess membrane permeation showed that HRGP induces lysis of E. faecalisbacteria in the presence of Zn2+ or at low pH. Heparin blocked binding of the protein to E. faecalis and abolished antibacterial activity. Furthermore, truncated HRGP, devoid of the heparin-binding and histidine-rich domain, was not antibacterial. It has previously been shown that peptides containing consensus heparin-binding sequences (Cardin and Weintraub motifs) are antibacterial. Thus, the peptide (GHHPH)4, derived from the histidine-rich region of HRGP and containing such a heparin-binding motif, was antibacterial for E. faecalis in the presence of Zn2+ or at low pH. The results show a previously undisclosed antibacterial activity of HRGP and suggest that the histidine-rich and heparin-binding domain of HRGP mediates the antibacterial activity of the protein.

Place, publisher, year, edition, pages
2007. Vol. 274, no 2, 377-389 p.
Keyword [en]
Antibacterial, Heparin, Histidine-rich glycoprotein, pH, Zinc
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-124682DOI: 10.1111/j.1742-4658.2006.05586.xISI: 000243234500010PubMedID: 17229145OAI: oai:DiVA.org:uu-124682DiVA: diva2:317863
Available from: 2010-05-05 Created: 2010-05-05 Last updated: 2017-12-12Bibliographically approved

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Olsson, Anna-Karin

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