Analysis of peptides using N-methylpolyvinylpyridium as silica surface modifier for CE-ESI-MS
2010 (English)In: Electrophoresis, ISSN 0173-0835, E-ISSN 1522-2683, Vol. 31, no 7, 1151-1156 p.Article in journal (Refereed) Published
In this study, the N-methylpolyvinylpyridinuim polymer has for the first time been used as a silica surface modifier for CE in combination with ESI MS (CE-ESI-MS). The compatibility for ESI-MS was demonstrated by the analysis of peptides and protein digests. The N-methylpolyvinylpyridium surface interacts electrostatically with the ionized silanol groups, giving a cationic surface with a reversed EOF. The surface modifier gave rapid and repeatable separations of peptides, proteins and protein digests at acidic pH for more than 4 h of continuous use. The CE separation yielded peak efficiencies of up to 4.3 x 10(5) plates/m. The surface coating is highly compatible with ESI and facilitates the separation and analysis of complex peptide mixtures as shown by the analysis of BSA digests.
Place, publisher, year, edition, pages
2010. Vol. 31, no 7, 1151-1156 p.
CE, ESI, MS, N-methylpolyvinylpyridinuim, peptides
IdentifiersURN: urn:nbn:se:uu:diva-125809DOI: 10.1002/elps.200900536ISI: 000276811000005PubMedID: 20209570OAI: oai:DiVA.org:uu-125809DiVA: diva2:321046