uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
The application and relevance of ex vivo culture systems for assessment of IBD treatment in murine models of colitis
Show others and affiliations
2008 (English)In: Pharmacological Research, ISSN 1043-6618, E-ISSN 1096-1186, Vol. 58, no 3-4, 222-231 p.Article in journal (Refereed) Published
Abstract [en]

The aim of this study was to investigate the relevance of mouse ex vivo cultures as a first screening model for new therapeutic agents of Inflammatory Bowel Disease (IBD). Two murine models (dextran sodium sulphate (DSS)-induced colitis and Galphai2-deficient mice) and two anti-inflammatory agents (methyl-prednisolone and the proteasome inhibitor MG132) were evaluated. The in vivo effects of methyl-prednisolone were assessed in both models. Ex vivo colonic tissue from both mouse models were cultured in the presence or absence of the drugs and TaqMan Low-Density arrays were used to assess the regulation of inflammatory genes before and after drug treatment. Colitis induced a similar inflammatory gene profile in both mouse models in in vivo studies and in ex vivo cultures. The differences encountered reflected the different phases of colitis in the models, e.g. innate cytokine/chemokine profile in the DSS model and T cell related markers in Galphai2-deficient mice. After steroid treatment, a similar pattern of genes was suppressed in the two mouse models. We confirmed the suppression of inflammatory gene expression for IL-1beta, IL-6 and iNOS in ex vivo and in vivo colons from both mouse models by quantitative RT-PCR. Importantly, the inflammatory responses in the murine ex vivo culture system reflected the in vivo response in the inflamed colonic tissue as assessed by changes in inflammatory gene expression, suggesting that the murine culture system can be used for validation of future IBD therapies.

Place, publisher, year, edition, pages
2008. Vol. 58, no 3-4, 222-231 p.
Keyword [en]
IBD, colitis
National Category
Gastroenterology and Hepatology Cell and Molecular Biology
Research subject
URN: urn:nbn:se:uu:diva-125820DOI: 10.1016/j.phrs.2008.08.006ISI: 000261131700008PubMedID: 18812224OAI: oai:DiVA.org:uu-125820DiVA: diva2:321062
Available from: 2010-05-28 Created: 2010-05-28 Last updated: 2010-07-13Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
By organisation
Department of Genetics and Pathology
In the same journal
Pharmacological Research
Gastroenterology and HepatologyCell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 189 hits
ReferencesLink to record
Permanent link

Direct link