Steroid profiles in ovarian follicular fluid in women with and without polycystic ovary syndrome, analyzed by liquid chromatography-tandem mass spectrometry.
2010 (English)In: Fertility and Sterility, ISSN 0015-0282, Vol. 94, no 6, 2228-2233 p.Article in journal (Refereed) Published
To compare steroid concentrations and steroid product-to-precursor ratios in ovarian follicular fluid (FF) from women with polycystic ovary syndrome (PCOS) and from regularly menstruating women in their early follicular phase, using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Polycystic ovary syndrome involves abnormal regulation of the steroidogenic enzymes, leading to arrest of follicle development.
University hospital clinic.
Follicular fluid from size-matched ovarian follicles (5-8 mm) in 27 nonstimulated women with PCOS and in 21 women without PCOS was sampled. Thirteen steroids were quantitated from 40 muL of FF, using LC-MS/MS.
MAIN OUTCOME MEASURE(S)
Concentrations of steroids in the FF and product-to-precursor ratios (enzyme activity) were compared between the groups.
In women with PCOS, ovarian FF contained higher concentrations of individual and total androgens, lower individual and total estrogens (E), and a lower total E-to-androgen ratio, compared with regularly menstruating women. The product-to-precursor concentration ratios indicated higher CYP17-linked and lower CYP19-linked (aromatase) enzyme activity. Receiver operating characteristic plots indicated the early CYP17 step (17-OH5P/5P) being highly important for the prevalence of PCOS (c = 0.95).
The women with PCOS had higher ovarian CYP17-linked and lower CYP19-linked (aromatase) enzyme activity, confirming previous data. Multiple steroid assessments from minute volumes including FF from nonstimulated ovaries, using LC-MS/MS, might be useful in research, clinical endocrinology, and in IVF.
Place, publisher, year, edition, pages
2010. Vol. 94, no 6, 2228-2233 p.
Androgens, CYP17, CYP19, estrogens, follicular fluid, LC-MS/MS, mass spectrometry, PCOS, Polycystic ovary syndrome, steroid hormones
Engineering and Technology Obstetrics, Gynecology and Reproductive Medicine Other Basic Medicine
IdentifiersURN: urn:nbn:se:uu:diva-126541DOI: 10.1016/j.fertnstert.2009.12.081ISI: 000283441400054PubMedID: 20171618OAI: oai:DiVA.org:uu-126541DiVA: diva2:324701
Published online 19 February 20102010-06-162010-06-162011-08-04Bibliographically approved