uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Analysis of small RNA in fission yeast; centromeric siRNAs are potentially generated through a structured RNA
Show others and affiliations
2009 (English)In: EMBO Journal, ISSN 0261-4189, E-ISSN 1460-2075, Vol. 28, no 24, 3832-3844 p.Article in journal (Refereed) Published
Abstract [en]

The formation of heterochromatin at the centromeres in fission yeast depends on transcription of the outer repeats. These transcripts are processed into siRNAs that target homologous loci for heterochromatin formation. Here, high throughput sequencing of small RNA provides a comprehensive analysis of centromere-derived small RNAs. We found that the centromeric small RNAs are Dcr1 dependent, carry 50-monophosphates and are associated with Ago1. The majority of centromeric small RNAs originate from two remarkably well-conserved sequences that are present in all centromeres. The high degree of similarity suggests that this non-coding sequence in itself may be of importance. Consistent with this, secondary structure-probing experiments indicate that this centromeric RNA is partially double-stranded and is processed by Dicer in vitro. We further demonstrate the existence of small centromeric RNA in rdp1D cells. Our data suggest a pathway for siRNA generation that is distinct from the well-documented model involving RITS/RDRC. We propose that primary transcripts fold into hairpin-like structures that may be processed by Dcr1 into siRNAs, and that these siRNAs may initiate heterochromatin formation independent of RDRC activity. The EMBO Journal (2009) 28, 3832-3844. doi: 10.1038/emboj.2009.351; Published online 26 November 2009

Place, publisher, year, edition, pages
2009. Vol. 28, no 24, 3832-3844 p.
Keyword [en]
centromeres, RNAi, small RNA, S. pombe
National Category
Biological Sciences
URN: urn:nbn:se:uu:diva-127373DOI: 10.1038/emboj.2009.351ISI: 000272833700006OAI: oai:DiVA.org:uu-127373DiVA: diva2:329716
Available from: 2010-07-13 Created: 2010-07-13 Last updated: 2014-05-28Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Wagner, Gerhart E. H.
By organisation
Department of Cell and Molecular Biology
In the same journal
EMBO Journal
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 151 hits
ReferencesLink to record
Permanent link

Direct link