Kcnq1ot1 noncoding RNA mediates transcriptional gene silencing by interacting with Dnmt1
2010 (English)In: Development, ISSN 0950-1991, E-ISSN 1477-9129, Vol. 137, no 15, 2493-2499 p.Article in journal (Refereed) Published
A long noncoding RNA, Kcnq1ot1, regulates the expression of both ubiquitously and tissue-specific imprinted genes within the Kcnq1 domain. However, the functional sequences of the Kcnq1ot1 RNA that mediate lineage-specific imprinting are unknown. Here, we have generated a knockout mouse with a deletion encompassing an 890-bp silencing domain (Delta890) downstream of the Kcnq1ot1 promoter. Maternal transmission of the Delta890 allele has no effect on imprinting, whereas paternal inheritance of the deletion leads to selective relaxation of the imprinting of ubiquitously imprinted genes to a variable extent in a tissue-specific manner. Interestingly, the deletion affects DNA methylation at somatically acquired differentially methylated regions (DMRs), but does not affect the histone modifications of the ubiquitously imprinted genes. Importantly, we found that Kcnq1ot1 recruits Dnmt1 to somatic DMRs by interacting with Dnmt1, and that this interaction was significantly reduced in the Delta890 mice. Thus, the ubiquitous and placental-specific imprinting of genes within the Kcnq1 domain might be mediated by distinct mechanisms, and Kcnq1ot1 RNA might mediate the silencing of ubiquitously imprinted genes by maintaining allele-specific methylation through its interactions with Dnmt1.
Place, publisher, year, edition, pages
2010. Vol. 137, no 15, 2493-2499 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-128879DOI: 10.1242/dev.048181ISI: 000279828300006PubMedID: 20573698OAI: oai:DiVA.org:uu-128879DiVA: diva2:332012