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HSA binding of HIV protease inhibitors: a high-performance affinity chromatography study
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Biochemistry and Organic Chemistry.
2009 (English)In: Journal of Separation Science, ISSN 1615-9306, E-ISSN 1615-9314, Vol. 32, no 10, 1625-1631 p.Article in journal (Refereed) Published
Abstract [en]

The binding of HIV protease inhibitors, drugs important for anti-HIV chemotherapy, to HSA was examined by high-performance affinity chromatography. Frontal analysis was first used to determine the amount of anchored protein and the binding capacity for selected markers on this column. Zonal elution experiments then ranked the HSA bound fraction of the examined compounds. Information on the g region was obtained by competitive zonal elution experiments using probe binding compounds with known sites on HSA. An allosteric competition between HIV protease inhibitors (PIs) and valproate (a probe for the bilirubin site) was detected, consistent with a noncooperative binding mechanism. No significant competition was observed between the examined compounds and salicylate or ibuprofen, probes for sites I and II, respectively. The observations were confirmed by circular dichroism spectroscopy, based on the change in the induced circular dichroism signals of selected markers for the main binding sites of HSA when ritonavir was added as the competitor. These results were in good agreement with previous literature reports and provide more details on how PIs are transported in plasma and how they may compete with other drugs in the body.

Place, publisher, year, edition, pages
2009. Vol. 32, no 10, 1625-1631 p.
National Category
Chemical Sciences
URN: urn:nbn:se:uu:diva-129017DOI: 10.1002/jssc.200900051ISI: 000266508700013OAI: oai:DiVA.org:uu-129017DiVA: diva2:332459
Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2010-08-26Bibliographically approved

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Danielson, Helena
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