uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Immunogenic males: a genome-wide analysis of reproduction and the cost of mating in Drosophila melanogaster females
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Evolution, Animal Ecology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Evolution, Animal Ecology.
2009 (English)In: Journal of Evolutionary Biology, ISSN 1010-061X, E-ISSN 1420-9101, Vol. 22, no 5, 964-973 p.Article in journal (Refereed) Published
Abstract [en]

In Drosophila melanogaster, mating radically transforms female physiology and behaviour. Post-mating responses include an increase in the oviposition rate, a reduction in female receptivity and an activation of the immune system. The fitness consequences of mating are similarly dramatic - females must mate once in order to produce fertile eggs, but additional matings have a clear negative effect. Previously, microarrays have been used to examine gene expression of females differing in their reproductive status with the aim of identifying genes influenced by mating. However, as only virgin and single mated females were compared, transcriptional changes associated with reproduction (under natural selection) and male-induced effects (possibly under sexually antagonistic selection) cannot be disentangled. We partitioned these fundamentally different effects by instead examining the expression profiles of virgin, single mated and double mated females. We found substantial effects relating to reproduction and further effects that are only attributable to mating itself. Immune response genes dominate this male-induced effect indicating that the cost of mating may be due partly to this system's activation. We propose that both sexually antagonistic and natural selection have been important in the evolution of the innate immunity genes, thereby contributing to the sexual dimorphism and rapid evolution at these loci.

Place, publisher, year, edition, pages
2009. Vol. 22, no 5, 964-973 p.
Keyword [en]
BIOCONDUCTOR, cost of mating, Drosophila melanogaster, innate immunity, microarrays, post-mating female response, sexual conflict, sperm competition
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-129120DOI: 10.1111/j.1420-9101.2009.01708.xISI: 000265252100005OAI: oai:DiVA.org:uu-129120DiVA: diva2:337683
Available from: 2010-08-09 Created: 2010-08-05 Last updated: 2017-12-12Bibliographically approved
In thesis
1. Sexual Conflict and Gene Expression in Drosophila melanogaster
Open this publication in new window or tab >>Sexual Conflict and Gene Expression in Drosophila melanogaster
2011 (English)Doctoral thesis, comprehensive summary (Other academic) [Artistic work]
Abstract [en]

Sexual conflict is broadly defined as a conflict between the evolutionary interests of the two sexes. Depending on the genetic architecture of the traits involved, it can occur at the level of male-female interactions or take the form of selection acting to change the mean of a shared trait against the sign of its genetic correlation. The aim of my thesis was to use genome-wide expression profiles in the model organism Drosophila melanogaster to provide novel insights in the study of sexual conflict.

First, we studied the female post-mating response to partition transcriptional changes associated with reproduction from male-induced effects, which are known to be harmful to females. We found substantial changes in expression of metabolic pathways associated with the activation of reproduction, while male-specific effects were dominated by the onset of an immune response. Changes in female response under different mating strategies was studied using experimental evolution: we found that monogamous females suffered decreased fecundity and their gene expression profiles suggested an overall weaker response to mating. To identify sexually antagonistic genes, we used hemiclonal lines and associated their sex-specific fitness with genome-wide transcript abundance. We confirmed the presence of a negative covariance for fitness and identified a group of candidate genes experiencing sexually antagonistic selection. We then focused on mitochondria, which can enable the accumulation of deleterious mutations with sex-specific effects due to their maternal inheritance, and found few effects on nuclear gene expression in females but major effects in males, predominantly in male-specific tissues. Finally, we used published data to compare intraspecific and interspecific genetic variation for a set of transcripts, to test whether speciation occurs along lines of maximum genetic variance.

In conclusion, gene expression techniques can generate useful results in the study of sexual conflict, particularly in association with phenotypic data or when integrated with published datasets.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2011. 46 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 843
Keyword
Sexual conflict, sexual selection, male-female coevolution, gene expression, transcriptome, microarrays, sexual dimorphism, Drosophila
National Category
Biological Sciences
Research subject
Biology with specialization in Animal Ecology; Biology with specialization in Evolutionary Genetics
Identifiers
urn:nbn:se:uu:diva-156567 (URN)978-91-554-8130-8 (ISBN)
Public defence
2011-10-01, Zootissalen, EBC, Villavägen 9, Uppsala, 09:30 (English)
Opponent
Supervisors
Available from: 2011-09-08 Created: 2011-08-03 Last updated: 2011-11-03

Open Access in DiVA

No full text

Other links

Publisher's full text
By organisation
Animal Ecology
In the same journal
Journal of Evolutionary Biology
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 363 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf