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Metabolic Adaptations in a H-2 Producing Heterocyst-Forming Cyanobacterium: Potentials and Implications for Biological Engineering
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Photochemistry and Molecular Science, Microbial Chemistry.
ChELSI Institute, Department of Chemical and Process Engineering, The University of Sheffield.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Photochemistry and Molecular Science, Microbial Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Photochemistry and Molecular Science, Microbial Chemistry.
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2011 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 10, no 4, 1772-1784 p.Article in journal (Refereed) Published
Abstract [en]

Nostoc punctiforme ATCC 29133 is a photoautotrophic cyanobacterium with the ability to fix atmospheric nitrogen and photoproduce hydrogen through the enzyme nitrogenase. The H-2 produced is reoxidized by an uptake hydrogenase. Inactivation of the uptake hydrogenase in N. punctiforme leads to increased H-2 release but unchanged rates of N-2 fixation, indicating redirected metabolism. System-wide understanding of the mechanisms of this metabolic redirection was obtained using complementary quantitative proteomic approaches, at both the filament and the heterocyst level. Of the total 1070 identified and quantified proteins, 239 were differentially expressed in the uptake hydrogenase mutant (NHM5) as compared to wild type. Our results indicate that the inactivation of uptake hydrogenase in N. punctiforme changes the overall metabolic equilibrium, affecting both oxygen reduction mechanisms in hetcrocysts as well as processes providing reducing equivalents for metabolic functions such as N-2 fixation. We identify specific metabolic processes used by NHM5 to maintain a high rate of N-2 fixation, and thereby potential targets for further improvement of nitrogenase based H-2 photogeneration. These targets include, but are not limited to, components of the oxygen scavenging capacity and cell envelope of heterocysts and proteins directly or indirectly involved in reduced carbon transport from vegetative cells to heterocysts.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2011. Vol. 10, no 4, 1772-1784 p.
Keyword [en]
iTRAQ, shotgun proteomics, Nostoc punctiforme ATCC 29133, uptake hydrogenase mutant, nitrogenase, dinitrogen fixation, heterocysts, NHMS, biotechnology
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-129227DOI: 10.1021/pr101055vISI: 000288924000031PubMedID: 21284387OAI: oai:DiVA.org:uu-129227DiVA: diva2:338003
Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2017-12-12

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Holmqvist, MarieStensjö, Karin

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