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Biotinylated lipid bilayer disks as model membranes for biosensor analyses
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Biochemistry and Organic Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Biochemistry and Organic Chemistry.
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2010 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 405, no 2, 153-159 p.Article in journal (Refereed) Published
Abstract [en]

The aim of this study was to investigate the potential of polyethylene glycol (PEG)-stabilized lipid bilayer disks as model membranes for surface plasmon resonance (SPR)-based biosensor analyses. Nanosized bilayer disks that included 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)(2000)] (DSPE-PEG(2000)-biotin) were prepared and structurally characterized by cryo-transmission electron microscopy (cryo-TEM) imaging. The biotinylated disks were immobilized via streptavidin to three different types of sensor chips (CM3, CM4, and CM5) varying in their degree of carboxymethylation and thickness of the dextran matrix. The bilayer disks were found to interact with and bind stably to the streptavidin-coated sensor surfaces. As a first step toward the use of these bilayer disks as model membranes in SPR-based studies of membrane proteins, initial investigations were carried out with cyclooxygenases 1 and 2 (COX 1 and COX 2). Bilayer disks were preincubated with the respective protein and thereafter allowed to interact with the sensor surface. The signal resulting from the interaction was, in both cases, significantly enhanced as compared with the signal obtained when disks alone were injected over the surface. The results of the study suggest that bilayer disks constitute a new and promising type of model membranes for SPR-based biosensor studies.

Place, publisher, year, edition, pages
2010. Vol. 405, no 2, 153-159 p.
Keyword [en]
Lipid bilayer disk, Liposome, Immobilization, Streptavidin, Biotin, Biacore, Surface plasmon resonance, SPR, Biosensor
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-129430DOI: 10.1016/j.ab.2010.06.030ISI: 000281296300002PubMedID: 20599649OAI: oai:DiVA.org:uu-129430DiVA: diva2:343708
Available from: 2010-08-15 Created: 2010-08-15 Last updated: 2017-12-12Bibliographically approved

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Nordström, HelenaDanielson, U HelenaEdwards, Katarina

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