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Absence of glutaredoxin1 increases lens susceptibility to oxidative stress induced by UVR-B
(Per Söderberg)
St. Erik's Eye Hospital, Karolinska Institutet. (Stefan Löfgren)
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2009 (English)In: Experimental Eye Research, ISSN 0014-4835, E-ISSN 1096-0007, Vol. 89, no 6, 833-839 p.Article in journal (Refereed) Published
Abstract [en]

We investigated if the absence of glutaredoxin1, a critical protein thiol repair enzyme, increases lens susceptibility to oxidative stress caused by in vivo exposure to ultraviolet radiation type B (UVR-B). Glrx(-/-) mice and Glrx(+/+) mice were unilaterally exposed in vivo to UVR-B for 15 min. Groups of 12 animals each received 4.3, 8.7, and 14.5 kJ/m(2) respectively. 48 h post UVR-B exposure, the induced cataract was quantified as forward lens light scattering. Cataract morphology was documented with darkfield illumination photography. Glutathione (GSH/GSSG) content was analyzed in Glrx(-/-) and Glrx(+/+) lenses. UVR-B exposure induced anterior sub-capsular cataract (ASC) in Glrx(-/-) and Glrx(+/+) mice. In Glrx(-/-) lenses the opacities extended further towards the lens equator than in wild type animals (Glrx(+/+)). Lens light scattering in Glrx(-/-) mice was increased in all dose groups compared to lenses with normal glutaredoxin1 function. The difference was more pronounced with increasing exposure dose. Lens sensitivity for UVR-B induced damage was significantly higher in Glrx(-/-) lenses compared to Glrx(+/+) lenses. The Glrx gene provides a 44% increase of protection against close to threshold UVR-B induced oxidative stress compared to the absence of the Glrx gene. In conclusion, the absence of glutaredoxin1 increases lens susceptibility to UVR-B induced oxidative stress in the mouse.

Place, publisher, year, edition, pages
2009. Vol. 89, no 6, 833-839 p.
Keyword [en]
cataract, lens light scattering, oxidative stress, ultraviolet radiation, glutaredoxin1, knockout mice, thioltransferase
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URN: urn:nbn:se:uu:diva-129448DOI: 10.1016/j.exer.2009.07.020ISI: 000272106000003PubMedID: 19664619OAI: oai:DiVA.org:uu-129448DiVA: diva2:343783
Ophthalmic Biophysics
Available from: 2010-08-16 Created: 2010-08-16 Last updated: 2013-11-22Bibliographically approved

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