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Selective insulin-like growth factor-I antagonist inhibits mouse embryo development in a dose-dependent manner
Department of Biosciences and Nutrition, Karolinska Institute, Sweden.
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2010 (English)In: Fertility and Sterility, ISSN 0015-0282, Vol. 93, no 8, 2621-2626 p.Article in journal (Refereed) Published
Abstract [en]

OBJECTIVE: To study the role of a synthetic insulin-like growth factor-I receptor (IGF-IR) antagonist, picropodophyllin, for mouse preimplantation embryo development in vivo and in vitro. DESIGN: In vitro and in vivo study. SETTING: Hospital-based research unit. ANIMALS: FVB/N mice and mouse embryos. INTERVENTION(S): The effect of picropodophyllin in mouse embryo development in vivo and in vitro, immunohistochemistry, ELISA, polymerase chain reaction. MAIN OUTCOME MEASURE(S): Embryo development, presence of IGF-IR, messenger RNA expression, IGF-I synthesis. RESULT(S): The effect of picropodophyllin on embryo development in vitro and in vivo was not reversible. Mice treated with picropodophyllin 1 to 3 days after mating had a reduced number of blastocysts, 40.5% versus 78.8%, and a higher number of embryos with delayed development, 48.6% versus 11.5%. Insulin-like growth factor-IR protein is present in both phosphorylated and nonphosphorylated form at all stages of embryo development. The relative IGF-IR messenger RNA expression was highest in the oocyte and reduced during development to blastocyst stage. Insulin-like growth factor-I in culture media was reduced after picropodophyllin treatment. CONCLUSION(S): We conclude that IGF-I has an important role in normal mouse embryo development and that its receptor plays an essential role in the embryonic genome activation process.

Place, publisher, year, edition, pages
2010. Vol. 93, no 8, 2621-2626 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-129616DOI: 10.1016/j.fertnstert.2009.12.044ISI: 000277608700022PubMedID: 20138270OAI: oai:DiVA.org:uu-129616DiVA: diva2:344532
Available from: 2010-08-19 Created: 2010-08-19 Last updated: 2010-12-17Bibliographically approved

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