Differential count of leukocytes A comparison between EPICS XL, Celldyn Sapphire and manual count by microscope
Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Differential count of leukocytes in blood (B-leukocytes) is an import analysis to diagnose blood diseases, infections and inflammatory diseases. B-Leukocyte are usually analysed by an automated haematology instrument that can determine the concentration of normal circulating white blood cells, neutrophil, eosinophil and basophil granulocytes, lymphocytes, and monocytes. If the automated cell count shows deviation such as unsure classification of cells, it should be complemented with manual count by microscopy. Manual count of leukocytes is time consuming, expensive and labour-intensive. By flow cytometry large numbers of samples can be analysed in a short time period and different types of blood cells can be identified in small samples. Flow cytometry is based on light scattering that depends on cell size and granularity of the cell. Flow cytometry also enables the measurement of fluorescence at several wavelengths, which is used when cells are labelled to cells with fluorescent antibodies. Antibodies used in this study were CD45, a marker of leukocytes, CD3 for T- lymphocytes, CD19 for B-lymphocytes, CD14 for monocytes, CD34 for haematopoietic stem cells and CD33 for myeloid cells.
In this study the flow cytometer EPICS XL was used to analyse patient samples and the results compared with that from the haematology instrument Celldyn Sapphire and manual
count by microscopy. The result showed high coefficients of correlation between EPICS XL and Celldyn Sapphire and agreed with result from manual count by microscopy.
Place, publisher, year, edition, pages
2010. , 24 p.
Flow cytometry, leukocyte differential count, blasts, antibodies, CD34.
IdentifiersURN: urn:nbn:se:uu:diva-129673OAI: oai:DiVA.org:uu-129673DiVA: diva2:344835
Douhan Håkansson, LenaLarsson, Anders