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Bovine binder-of-sperm protein BSP1 promotes protrusion and nanotube formation from liposomes
Department of Chemistry, Université de Montréal, Canada.
Department of Chemistry, Université de Montréal, Canada.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Physical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Physical Chemistry.
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2010 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 399, no 3, 406-411 p.Article in journal (Refereed) Published
Abstract [en]

Binder-of-sperm (BSP) proteins interact with sperm membranes and are proposed to extract selectively phosphatidylcholine and cholesterol from these. This change in lipid composition is a key step in sperm capacitation. The present work demonstrates that the interactions between the protein BSP1 and model membranes composed with phosphatidylcholine lead to drastic changes in the morphology of the lipidic self-assemblies. Using cryo-electron microscopy and fluorescence microscopy, we show that, in the presence of the protein, the lipid vesicles elongate, and form bead necklace-like structures that evolve toward small vesicles or thread-like structures. In the presence of multilamellar vesicles, where a large reservoir of lipid is available, the presence of BSP proteins lead to the formation of long nanotubes. Long spiral-like threads, associated with lipid/protein complexes, are also observed. The local curvature of lipid membranes induced by the BSP proteins may be involved in lipid domain formation and the extraction of some lipids during the sperm maturation process.

Place, publisher, year, edition, pages
2010. Vol. 399, no 3, 406-411 p.
Keyword [en]
Binder-of-sperm (BSP) proteins, Sperm, Membranes, Protrusions, Nanotubes, Fluorescence microscopy, Cryo-electron microscopy, Lipids, Lipid curvature, Lipid morphology
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-130073DOI: 10.1016/j.bbrc.2010.07.088ISI: 000281587400017PubMedID: 20674552OAI: oai:DiVA.org:uu-130073DiVA: diva2:346211
Available from: 2010-08-31 Created: 2010-08-31 Last updated: 2017-12-12Bibliographically approved

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Karlsson, GöranEdwards, Katarina

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