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Real-time immunohistochemistry analysis of embedded tissue
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Oncology, Radiology and Clinical Immunology, Biomedical Radiation Sciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Oncology, Radiology and Clinical Immunology, Biomedical Radiation Sciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Oncology, Radiology and Clinical Immunology, Biomedical Radiation Sciences.
2010 (English)In: Applied Radiation and Isotopes, ISSN 0969-8043, E-ISSN 1872-9800, Vol. 68, no 12, 2372-2376 p.Article in journal (Refereed) Published
Abstract [en]

We present a novel analysis of membrane-protein expression in tissue sections based on semi-automatic real-time measurement using LigandTracer(®) technology. A commercial antiHER2 antibody developed for immunohistochemistry used in this setup was revealed to have sub-optimal interaction with tissue when analyzed as recommended for immunohistochemistry. We therefore think that real-time measurement of tissue, offering direct and quantitative membrane-protein interaction analysis, can lead to improved reproducibility and eliminate the subjective operator dependences that classical immunohistochemsitry suffers from.

Place, publisher, year, edition, pages
2010. Vol. 68, no 12, 2372-2376 p.
Keyword [en]
Immunohistochemistry, IHC, Antibody, Kinetics, Tissue, Real-time
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-130176DOI: 10.1016/j.apradiso.2010.06.003ISI: 000283384400039PubMedID: 20828661OAI: oai:DiVA.org:uu-130176DiVA: diva2:347425
Available from: 2010-09-03 Created: 2010-09-03 Last updated: 2017-12-12Bibliographically approved

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