Real-time immunohistochemistry analysis of embedded tissue
2010 (English)In: Applied Radiation and Isotopes, ISSN 0969-8043, E-ISSN 1872-9800, Vol. 68, no 12, 2372-2376 p.Article in journal (Refereed) Published
We present a novel analysis of membrane-protein expression in tissue sections based on semi-automatic real-time measurement using LigandTracer(®) technology. A commercial antiHER2 antibody developed for immunohistochemistry used in this setup was revealed to have sub-optimal interaction with tissue when analyzed as recommended for immunohistochemistry. We therefore think that real-time measurement of tissue, offering direct and quantitative membrane-protein interaction analysis, can lead to improved reproducibility and eliminate the subjective operator dependences that classical immunohistochemsitry suffers from.
Place, publisher, year, edition, pages
2010. Vol. 68, no 12, 2372-2376 p.
Immunohistochemistry, IHC, Antibody, Kinetics, Tissue, Real-time
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-130176DOI: 10.1016/j.apradiso.2010.06.003ISI: 000283384400039PubMedID: 20828661OAI: oai:DiVA.org:uu-130176DiVA: diva2:347425