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Altered mRNA-expression due to acute mesenteric ischaemia in a porcine model
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Vascular Surgery.
Sektionen för klinisk kemi, Örebro Universitetssjukhus.
Kärlcentrum, Malmö Universitetssjukhus.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Vascular Surgery.
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2011 (English)In: European Journal of Vascular and Endovascular Surgery, ISSN 1078-5884, E-ISSN 1532-2165, Vol. 41, no 2, 281-287 p.Article in journal (Refereed) Published
Abstract [en]

Introduction: mRNA changes in the small intestine in response to acute mesenteric ischaemia (AMI) could offer novel diagnostic possibilities, but have not been described. The aim was to characterize the mRNA response to experimental AMI.

Materials and methods: Twelve pigs underwent catheterization of the superior mesenteric artery with injection of polivinylalcohol embolization particles or sodium chloride. Laparotomy and intestinal tissue sampling was performed. Microarray analysis was performed using the GeneChip® whole porcine genome array.

Results: Seven down-regulated cellular pathways were associated with protein, lipid and carbohydrate metabolism. Seventeen up-regulated pathways were associated with inflammatory and immunological activity, regulation of extracellular matrix and decreased cellular proliferation. Thrombospondin(THS), Monocyte Chemoattractant Protein 1(MCP-1) and Gap Junction Alpha 1(GJA-1) were consistently up-regulated in all embolized pigs. Genes encoding earlier proposed biomarkers for AMI were up-regulated, such as lactate dehydrogenase and creatine kinase, or down-regulated, such as intestinal fatty acid binding protein and glutathione S-transferase.

Conclusion: This study describes the intestinal tissue response on a gene expression level to AMI. THS, MCP-1 and GJA-1 were consistently up-regulated by ischemia, whereas earlier proposed biomarkers for AMI were not. Gene expression may not be directly linked to the use of the corresponding proteins as potential clinical biomarkers.

Place, publisher, year, edition, pages
2011. Vol. 41, no 2, 281-287 p.
Keyword [en]
Superior mesenteric artery, Acute mesenteric ischaemia, Gene expression analysis, Porcine, Microarray
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-130813DOI: 10.1016/j.ejvs.2010.09.012ISI: 000288469000022PubMedID: 21095140OAI: oai:DiVA.org:uu-130813DiVA: diva2:351321
Available from: 2010-09-13 Created: 2010-09-13 Last updated: 2017-12-12Bibliographically approved

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Block, TomasBjörck, Martin

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