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MicroRNAs in Amoebozoa: Deep sequencing of the small RNA population in the social amoeba Dictyostelium discoideum reveals developmentally regulated microRNAs
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Science for Life Laboratory, SciLifeLab.
2012 (English)In: RNA: A publication of the RNA Society, ISSN 1355-8382, E-ISSN 1469-9001, Vol. 18, no 10, 1771-1782 p.Article in journal (Refereed) Published
Abstract [en]

The RNA interference machinery has served as a guardian of eukaryotic genomes since the divergence from prokaryotes. Although the basic components have a common origin, silencing pathways directed by small RNAs have evolved in diverse directions in different eukaryotic lineages. One example is miRNAs. Their regulation of protein coding genes has been shown to play a vital role in plants and animals but little is known about their role in other organisms. The single cell social amoeba Dictyostelium discoideum could hold the answers to some questions regarding the evolution and function of small RNA pathways. Here we report deep sequencing of small RNAs from three developmental stages of D. discoideum. Analyses of these libraries as well as experimental data reveal the expression of a number of miRNAs, several which have distinct expression patterns during development. We also find miRNAs processed from a hairpin originating from a repetitive element that we believe could represent a pathway for the generation of new miRNAs.

Place, publisher, year, edition, pages
2012. Vol. 18, no 10, 1771-1782 p.
Keyword [en]
microRNA, Dictyostelium discoideum, high-throughput sequencing, small RNA, development
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-131165DOI: 10.1261/rna.033175.112ISI: 000309002700003OAI: oai:DiVA.org:uu-131165DiVA: diva2:353366
Available from: 2010-09-26 Created: 2010-09-26 Last updated: 2017-12-12Bibliographically approved

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Wagner, Gerhart Eduard HeinrichSöderbom, Fredrik

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