Determination of melatonin in saliva using automated solid-phase extraction, high-performance liquid chromatography and fluorescence detection
2006 (English)In: Scandinavian Journal of Clinical and Laboratory Investigation, ISSN 0036-5513, Vol. 66, no 3, 181-190 p.Article in journal (Refereed) Published
A sensitive bioanalytical method for the determination of melatonin in saliva by solid‐phase extraction (SPE), high‐performance liquid chromatography (HPLC) and fluorescence detection has been developed and validated. Saliva was collected with a Salivette® sampling device (Sarstedt) and a mixed‐mode SPE column was used for the extraction of melatonin and internal standard (N‐acetyl‐6‐methoxytryptamine) from the saliva. Chromatographic separation was performed using a HyPurity C18 LC column (150×2.1mm) with mobile phase acetonitrile – ammonium hydrogen carbonate buffer, 0.015M, pH6.8 (23:77, v/v). Excitation and emission wavelengths were set to 285nm and 345nm, respectively. The within‐day precision for the method at 50pmol/L was 7.9 % and the between‐day precision was 10.5 %. The limit of quantification was 50pmol/L.
Place, publisher, year, edition, pages
2006. Vol. 66, no 3, 181-190 p.
Circadian rhythm, HPLC, indoleamine, N-acetyl-5-methoxytryptamine, Salivette, SPE
Other Basic Medicine Chemical Sciences
IdentifiersURN: urn:nbn:se:uu:diva-131515DOI: 10.1080/00365510600548777OAI: oai:DiVA.org:uu-131515DiVA: diva2:354649