Determination of eflornithine enantiomers in plasma by precolumn derivatization with o-phthalaldehyde-N-acetyl-l-cysteine and liquid chromatography with UV detection
2010 (English)In: BMC. Biomedical chromotography, ISSN 0269-3879, E-ISSN 1099-0801, Vol. 24, no 7, 768-773 p.Article in journal (Refereed) Published
A bioanalytical method for indirect determination of eflornithine enantiomers in 75 mu L human plasma has been developed and validated. L- and D-eflornithine were derivatized with o-phthalaldehyde and N-acetyl-L-cysteine to generate diastereomers which were separated on two serially connected Chromolith Performance columns (RP-18e 100 x 4.6 mm i.d.) by a isocratic flow followed by a gradient flow for elution of endogenous compounds. The diastereomers were detected with UV (340 nm). The between-day precisions for L- and D-eflornithine in plasma were 8.4 and 2.3% at 3 mu m, 4.0 and 5.1% at 400 mu m, and 2.0 and 3.7% at 1000 mu m. The lower limit of quantification was determined to be 1.5 mu m, at which precision was 14.9 and 9.9% for 1- and D-eflornithine, respectively.
Place, publisher, year, edition, pages
2010. Vol. 24, no 7, 768-773 p.
eflornithine, chiral separation, Human African sleeping sickness, cancer
Other Basic Medicine Chemical Sciences
IdentifiersURN: urn:nbn:se:uu:diva-131517DOI: 10.1002/bmc.1361ISI: 000279367900013OAI: oai:DiVA.org:uu-131517DiVA: diva2:354652