Expression of ghrelin and obestatin in neuroendocrine tumors
(English)Manuscript (preprint) (Other academic)
Introduction: The peptide hormones ghrelin and obestatin are derived from the same hormone precursor. Ghrelin, which is mainly produced by the gastric mucosa, is involved in many biological processes ranging from growth hormone release to appetite regulation. Obestatin has an identical distribution as ghrelin but its physiological function still needs further investigation. The aim of the present study was to elucidate the incidence of ghrelin- and obestatin-immunoreactive cells in a comprehensive panel of human neuroendocrine tumors (NETs).
Material and Methods: Immunohistochemistry with polyclonal antibodies vs. ghrelin and obestatin was used to investigate the expression of the two peptides in NETs (n=128) and other endocrine-related disorders (n=3). Co-expression of the peptides was evaluated by double-immunofluorescence.
Results: Ghrelin and obestatin were expressed in 13/128 and 14/128 tumor tissues respectively, mainly representing NETs of foregut and less of hindgut origin. Double immunofluorescence staining showed co-localization of the peptides. Ghrelin and obestatin were also detected in pancreatic tissue from a nesiodioblastosis patient.
Conclusion: A minority of NETs, mainly of foregut origin, express ghrelin and obestatin. Our results indicate that these hormones should not be included in the general work-up of patients with NETs. The significance of the few ghrelin and obestatin immunoreactive tumor cells has to be further explored.
obestatin, ghrelin, neuroendocrine tumors, immunohistochemistry
Medical and Health Sciences
Research subject Endocrinology and Diabetology
IdentifiersURN: urn:nbn:se:uu:diva-129501OAI: oai:DiVA.org:uu-129501DiVA: diva2:354735
ProjectsExpression of neuroendocrine markers in normal and neoplastic tissue with an emphasis on ghrelin and obestatin