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Novel natural peptides from Hyla arborea schelkownikowi skin secretion
Organic Chemistry Department, Moscow State University.
Organic Chemistry Department, Moscow State University.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Analytical Chemistry.
N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medicial Sciences.
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2010 (English)In: Rapid Communications in Mass Spectrometry, ISSN 0951-4198, E-ISSN 1097-0231, Vol. 24, no 12, 1749-1754 p.Article in journal (Refereed) Published
Abstract [en]

Hyla arborea schelkownikowi is one of the leaf frog species inhabiting the southern territories of Russia and the former USSR. This frog species is a member of the Hylidae Rafinesque, 1815 batrachians family. The present study deals with the previously uninvestigated peptidome of the Hyla arborea schelkownikowi skin secretion. Nano-electrospray ionization Fourier transform mass spectrometry (nanoESI-FTMS) of the skin secretion, in the intact form and after acetylation, was selected as the general method of analysis. Electron-capture dissociation (ECD) and collision-induced dissociation (CID) fragmentation were both employed, while de novo sequencing was performed by manual interpretation of the MS data. The suppression of the cyclization of b-ions in the mass spectrometer by the acetylation reaction proved to be very efficient for the de novo sequencing of short peptides. Ten skin peptides were found and all of them, except for bradykinin, had not previously been reported. Six of the peptides belong to the tryptophyllins and related peptides, while three peptides are similar to the aureins.

Place, publisher, year, edition, pages
John Wiley & Sons, Ltd. , 2010. Vol. 24, no 12, 1749-1754 p.
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Medical and Health Sciences Analytical Chemistry
URN: urn:nbn:se:uu:diva-131605DOI: 10.1002/rcm.4571ISI: 000278661400005PubMedID: 20499319OAI: oai:DiVA.org:uu-131605DiVA: diva2:355196
Available from: 2010-10-07 Created: 2010-10-06 Last updated: 2010-12-28Bibliographically approved

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Artemenko, Konstantin A.Zubarev, Roman A.
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