uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Fetal pancreatic islets express functional leptin receptors and leptin stimulates proliferation of fetal islet cells
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Internal Medicine.
2000 (English)In: International Journal of Obesity, ISSN 0307-0565, E-ISSN 1476-5497, Vol. 24, no 10, 1246-1253 p.Article in journal (Refereed) Published
Abstract [en]

OBJECTIVE: Previous studies have demonstrated that leptin can stimulate proliferation of insulin-secreting tumor cell lines. The objective of this study was to characterize whether leptin could stimulate proliferation of primary beta-cells too. Since adult beta-cells have very limited capacity for replication, we examined the effect of leptin on islets of Langerhans obtained from fetal rats, in a tissue culture system. METHODS: Leptin receptor mRNA and c-fos mRNA were measured by RT-PCR. Proliferation of fetal rat islet cells was measured by a WST-1 colorimetric assay and [3H]-thymidine incorporation assay. RESULTS: Leptin stimulated proliferation of serum-deprived fetal rat islet cells, as indicated by increased formation of formazan dye from a tetrazolium salt WST-1. Leptin stimulated DNA synthesis in islet cells, as indicated by increased [3H]-thymidine incorporation into DNA. The effect of leptin on islet cell proliferation was on average 39-50% of the effect obtained with 10% fetal bovine serum. Leptin increased c-fos mRNA expression by 2.8-fold in isolated fetal islets after 30 min treatment. In fetal pancreatic islets, both the common extracellular portion (OB-R) and the intact long form (OB-Rb) of the leptin receptor were readily detected by reverse transcriptase polymerase chain reaction. CONCLUSION: Functional leptin receptors are expressed in pancreatic islet cells, as early as during the fetal stage of development of these microorgans. Leptin stimulates proliferation of fetal islet cells and might play a role in determining islet cell mass at birth.

Place, publisher, year, edition, pages
2000. Vol. 24, no 10, 1246-1253 p.
Keyword [en]
leptin, insulin-secreting cells, islets of Langerhans, fetal islets
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-132286PubMedID: 11093284OAI: oai:DiVA.org:uu-132286DiVA: diva2:357452
Available from: 2010-10-18 Created: 2010-10-18 Last updated: 2010-10-26Bibliographically approved

Open Access in DiVA

No full text

By organisation
Internal Medicine
In the same journal
International Journal of Obesity
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 184 hits
ReferencesLink to record
Permanent link

Direct link