uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Ryanodine receptor-operated activation of TRP-like channels can trigger critical Ca2+ signaling events in pancreatic beta-cells
Show others and affiliations
2005 (English)In: The FASEB Journal, ISSN 0892-6638, E-ISSN 1530-6860, Vol. 19, no 2, 301-303 p.Article in journal (Refereed) Published
Abstract [en]

There is little information available concerning the link between the ryanodine (RY) receptors and the downstream Ca(2+) signaling events in beta-cells. In fura-2 loaded INS-1E cells, activation of RY receptors by 9-methyl 5,7-dibromoeudistomin D (MBED) caused a rapid rise of [Ca(2+)]i followed by a plateau and repetitive [Ca(2+)]i spikes on the plateau. The [Ca(2+)]i plateau was abolished by omission of extracellular Ca(2+) and by SKF 96365. In the presence of SKF 96365, MBED produced a transient increase of [Ca(2+)]i, which was abolished by thapsigargin. Activation of RY receptors caused Ca(2+) entry even when the ER Ca(2+) pool was depleted by thapsigargin. The [Ca(2+)]i plateau was not inhibited by nimodipine or ruthenium red, but was inhibited by membrane depolarization, La(3+), Gd(3+), niflumic acid, and 2-aminoethoxydiphenyl borate, agents that inhibit the transient receptor potential channels. The [Ca(2+)]i spikes were inhibited by nimodipine and ryanodine, indicating that they were due to Ca(2+) influx through the voltage-gated Ca(2+) channels and Ca(2+)-induced Ca(2+) release (CICR). Activation of RY receptors depolarized membrane potential as measured by patch clamp. Thus, activation of RY receptors leads to coherent changes in Ca(2+) signaling, which includes activation of TRP-like channels, membrane depolarization, activation of the voltage-gated Ca(2+) channels and CICR.

Place, publisher, year, edition, pages
2005. Vol. 19, no 2, 301-303 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-132280DOI: 10.1096/fj.04-2621fjePubMedID: 15572434OAI: oai:DiVA.org:uu-132280DiVA: diva2:357459
Available from: 2010-10-18 Created: 2010-10-18 Last updated: 2015-08-12Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Lund, Per-Eric
By organisation
Internal Medicine
In the same journal
The FASEB Journal
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 145 hits
ReferencesLink to record
Permanent link

Direct link