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Association of cystatin C with prostasomes in human seminal plasma
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Chemistry. (biologisk struktur och funktion)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Chemistry. (biologisk struktur och funktion)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Chemistry. (biologisk struktur och funktion)
The Andrology Laboratory at Queen Sophia Hospital, Stockholm, Sweden. (biologisk struktur och funktion)
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2011 (English)In: International Journal of Andrology, ISSN 0105-6263, E-ISSN 1365-2605, Vol. 33, no 4, 363-368 p.Article in journal (Refereed) Published
Abstract [en]

It was recently elucidated that cystatin C, a protein targeted to the classical secretory pathway by its signal peptide sequence, can also be secreted in association with exosomes. Accordingly, we wanted to investigate whether there is a secretory link between cystatin C and prostasomes in human seminal plasma. Cystatin C concentrations in seminal plasma from 50 men including 6 vasectomized men were measured by turbidimetry on an Architect Ci8200. Some of the seminal plasma samples were also analysed utilizing an Epics Profile XL-MCL cytometer. We found high concentrations of cystatin C in seminal plasma. The 2.5-97.5 percentiles, performed by bootstrap estimation, were 25.8 [95% confidence interval (CI): 22.3-29.4] to 77.0 mg/L (95% CI: 71.9-82.1). Cystatin C is present in approximately 50 times higher concentration in seminal plasma compared with blood plasma. There was no clear difference as regards seminal plasma content of cystatin C between vasectomized men and the rest of the group. Immunoblot analysis with chicken anti-cystatin C antibody revealed a firm association of cystatin C with prostasomes. Flow cytometric analysis demonstrated that cystatin C was linked to prostasomes also meaning an at least partial prostasomal membrane surface localization.

Place, publisher, year, edition, pages
2011. Vol. 33, no 4, 363-368 p.
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Medical and Health Sciences
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URN: urn:nbn:se:uu:diva-132558DOI: 10.1111/j.1365-2605.2010.01090.xISI: 000292889200009PubMedID: 20609026OAI: oai:DiVA.org:uu-132558DiVA: diva2:358285
Available from: 2010-10-21 Created: 2010-10-21 Last updated: 2017-12-12Bibliographically approved

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