Effects of the HDAC inhibitor valproic acid on human pericytes in vitro
(English)Manuscript (preprint) (Other academic)
Microvasculare pericytes are of key importance in neoformation of blood vessels, in stabilization of newly formed vessels as well as maintenance of angiostasis in resting tissues. In the present study the effects of the HDAC inhibitor VPA on pericyte proliferation, migration and differentiation was investigated. Furthermore, expression of genes known to be involved in different aspects of angiogenesis were investigated in primary pericyte cultures as well as the effects of VPA on the expression of these set of genes using quantative PCR arrays. The results show that HDAC inhibition leads to inhibition of pericyte proliferation and migration as well as pericyte differentiation into collagen type I producing fibroblasts. Furthermore, HDAC inhibition promoted expression of mRNAs of genes involved in vessel stabilization/maturation in human microvascular pericytes. The present study suggests that VPAs effect on angiogenesis via microvascular pericytes is through vessel stabilization.
pericyte HDACinhibitor VPA
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) Cell and Molecular Biology
Research subject Biochemistry; Cell Research; Medical Biochemistry; Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-132608OAI: oai:DiVA.org:uu-132608DiVA: diva2:359879
De två sista författarna delar förstaförfattarskapet2010-11-012010-10-222013-04-08