Can DNA sampling from the rectal mucosa be a novel tool for the detection of colorectal cancer?
2010 (English)In: International Journal of Colorectal Disease, ISSN 0179-1958, E-ISSN 1432-1262, Vol. 25, no 9, 1071-1078 p.Article in journal (Refereed) Published
INTRODUCTION: The objective was to evaluate a new method for DNA sampling from the rectal mucosa for the detection of colorectal cancer or any clinically significant pathology in the colon and rectum. METHODS: This prospective cohort study included patients scheduled for colonoscopy (group 1, n = 185) or colonic resection because of suspected colorectal cancer (group 2, n = 62). A test instrument with a balloon-holding end was introduced through a proctoscope into the rectum to collect exfoliated cells, from which DNA was isolated and quantified. RESULTS: The detection of colorectal cancer in group 1 showed a sensitivity for the DNA cut-off levels 1.5, 2, and 2.5 microg/ml of 100%, 80%, and 60%, and a specificity of 37%, 46%, and 56%, respectively. In group 2, for the same cut-off levels, the sensitivity was 73%, 61%, and 55%, and the specificity was 67%, 67%, and 67%, respectively. CONCLUSIONS: This novel technique is a safe and easy way of collecting DNA from the rectal mucosa. The sensitivity and specificity of the test were too low to be acceptable for a screening test. The low sensitivity and specificity in this study could be explained by the diversity within the study groups as many patients presented with long-term history of colorectal disease and surgical interventions in the past.
Place, publisher, year, edition, pages
2010. Vol. 25, no 9, 1071-1078 p.
Colon cancer, Rectal cancer, Screening, Fecal DNA, Exfoliated colonocyte collection
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-133091DOI: 10.1007/s00384-010-0979-5ISI: 000280578500005PubMedID: 20563588OAI: oai:DiVA.org:uu-133091DiVA: diva2:360084