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TGF-beta Signaling in Gingival Fibroblast-Epithelial Interaction
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
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2010 (English)In: Journal of Dental Research, ISSN 0022-0345, E-ISSN 1544-0591, Vol. 89, no 11, 1315-1321 p.Article in journal (Refereed) Published
Abstract [en]

The underlying mechanism and the therapeutic regimen for the transition of reversible gingivitis to irreversible periodontitis are unclear. Since transforming growth factor (TGF)-beta has been implicated in differentially regulated gene expression in gingival fibroblasts, we hypothesized that TGF-beta signaling is activated in periodontitis-affected gingiva, along with enhanced collagen degradation, that is reversed by TGF-beta inhibition. A novel three-dimensional (3D) gel-culture system consisting of primary human gingival fibroblasts (GF) and gingival epithelial (GE) cells in collagen gels was applied. GF populations from patients with severe periodontitis degraded collagen gels, which was reduced by TGF-beta-receptor kinase inhibition. Up-regulation of TGF-beta-responsive genes was evident in GF/GE cocultures. Furthermore, the TGF-beta downstream transducer Smad3C was highly phosphorylated in periodontitis-affected gingiva and 3D cultures. These results imply that TGF-beta signaling is involved in fibroblast-epithelial cell interaction in periodontitis, and suggest that the 3D culture system is a useful in vitro model for therapeutic drug screening for periodontitis.

Place, publisher, year, edition, pages
2010. Vol. 89, no 11, 1315-1321 p.
Keyword [en]
periodontitis, TGF-beta signaling, extracellular matrix degradation, three-dimensional (3D) culture, human gingival fibroblasts
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URN: urn:nbn:se:uu:diva-133605DOI: 10.1177/0022034510378423ISI: 000283363300025OAI: oai:DiVA.org:uu-133605DiVA: diva2:369989
Available from: 2010-11-12 Created: 2010-11-11 Last updated: 2010-11-12Bibliographically approved

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