Sensitive Detection of Bacterial DNA by Magnetic Nanoparticles
2010 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 82, no 22, 9138-9140 p.Article in journal, Letter (Refereed) Published
This work presents sensitive detection of bacterial genomic DNA using a magnetic nanoparticle-based substrate-free method. For the first time, such a method is employed for detection of a clinically relevant analyte by implementing a solid-phase-based molecular probing and amplification protocol that can be executed in 80 min. The molecular detection and amplification protocol is presented and verified on samples containing purified genomic DNA from Escherichia coli cells, showing that as few as 50 bacteria can be detected. This study moves the use of volume-amplified magnetic nanoparticles one step further toward rapid, sensitive, and selective infectious diagnostics.
Place, publisher, year, edition, pages
2010. Vol. 82, no 22, 9138-9140 p.
Analytical Chemistry Other Materials Engineering
Research subject Engineering Science with specialization in Nanotechnology and Functional Materials
IdentifiersURN: urn:nbn:se:uu:diva-133693DOI: 10.1021/ac102133eISI: 000284080500002OAI: oai:DiVA.org:uu-133693DiVA: diva2:370069