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Plasma concentrations of corticosterone and buprenorphine in rats subjected to jugular vein catheterization
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Comparative Medicine.
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2010 (English)In: Laboratory Animals. Journal of the Laboratory Animal Science Association, ISSN 0023-6772, Vol. 44, no 4, 337-343 p.Article in journal (Refereed) Published
Abstract [en]

The present study investigated the postoperative plasma concentrations of corticosterone and buprenorphine in male Wistar and Sprague-Dawley rats, treated with buprenorphine administered either through subcutaneous (SC) injection or through voluntary ingestion (VI). The animals were treated with buprenorphine for pre-emptive analgesia prior to surgical placement of a jugular catheter, followed by automated blood sampling during 96 h. Buprenorphine was administered on a regular basis throughout the experiment, and blood was collected on selected time points. Body weight was measured before and 96 h after surgery. It was found that the two rat stocks responded in a similar manner to both buprenorphine treatments, with the exception of body weight change in Wistar rats, in which body weight was reduced after SC treatment. The plasma concentration of corticosterone was significantly higher in the SC-treated animals than in the VI-treated animals during the first 18 h of the study, while plasma buprenorphine concentration was at least as high and more even over time after VI treatment. The present study shows that buprenorphine administration through VI is suitable for both Wistar and Sprague-Dawley rats, with lower stress response and higher plasma concentrations of buprenorphine than after the traditional SC route of administration.

Place, publisher, year, edition, pages
2010. Vol. 44, no 4, 337-343 p.
Keyword [en]
Rats, catheterization, analgesia, buprenorphine, surgical stress, corticosterone
National Category
Biomedical Laboratory Science/Technology
URN: urn:nbn:se:uu:diva-133727DOI: 10.1258/la.2010.009115ISI: 000283457500008OAI: oai:DiVA.org:uu-133727DiVA: diva2:370831
Available from: 2010-11-18 Created: 2010-11-15 Last updated: 2013-02-11Bibliographically approved
In thesis
1. Surgical Stress in Rats: The Impact of Buprenorphine on Postoperative Recovery
Open this publication in new window or tab >>Surgical Stress in Rats: The Impact of Buprenorphine on Postoperative Recovery
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

During surgery, both anesthesia and tissue damage cause physiological stress responses in the body. The hypothalamic-pituitary-adrenal (HPA) axis is activated with increased levels of glucocorticoids. After surgical procedures the stress response may be a cause of postoperative morbidity and pre-emptive analgesic treatment can attenuate the stress response during the postoperative period. In laboratory animals, buprenorphine is a commonly used analgesic. Subcutaneous (s.c.) administration of buprenorphine is most common, but oral administration would be preferable in many cases, enabling administration without any handling of the rat.

In this thesis we studied the surgical stress response in laboratory rats during surgery and in the postoperative period, and its modulation by s.c. injection and oral voluntary ingestion (VI) of buprenorphine. Corticosterone levels and the clinical parameters body weight, water intake and behavior were observed. The concentration of buprenorphine in plasma was measured as well as stock-related differences in postoperative recovery.

During surgery and anesthesia there was a higher corticosterone release during a more severe surgery and corticosterone levels were reduced more effectively after buprenorphine treatment than after lidocaine treatment.

Buprenorphine treatment, independent of the route of administration, led to better postoperative recovery in body weight and water intake compared to local anesthetics. VI of buprenorphine resulted in a suppression of plasma corticosterone levels compared to s.c. buprenorphine treatment and treatment with local anesthetics during the first day after surgical catheterization. The corticosterone levels of all buprenorphine treated groups had, by the second postoperative day, reverted to the normal diurnal rhythm of corticosterone secretion. Buprenorphine treatment increased locomotor activity in non-operated rats only. The effect of buprenorphine in operated rats could not be detected via the monitoring of locomotor activity or the time spent resting in the present study.

Treatment with buprenorphine by VI has similar effects on postoperative plasma corticosterone levels in both Wistar and Sprague-Dawley rats. VI of buprenorphine resulted in a buprenorphine concentration in plasma at least as high as by s.c. treatment.

Thus, administration by VI of buprenorphine appears to be an effective stress-reducing method for administrating postoperative analgesia to laboratory rats.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2013. 66 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 849
Surgical stress, Corticosterone, Buprenorphine, Analgesia, Rats.
National Category
Other Basic Medicine
Research subject
Comparative Medicine
urn:nbn:se:uu:diva-185686 (URN)978-91-554-8558-0 (ISBN)
Public defence
2013-02-01, BMC, B22, Husargatan 3, Uppsala, 09:15 (Swedish)
Available from: 2013-01-11 Created: 2012-11-27 Last updated: 2013-04-02

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