Filtration assay for arachidonic acid release
2010 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 407, no 2, 233-236 p.Article in journal (Refereed) Published
Arachidonic acid (AA) release is a central message in cell signaling. Fatty acid release is generally assessed by manual sampling of radioactivity release from cells prelabeled with a radiolabeled fatty acid. The assay is laborious, time-consuming, and susceptible to high noise. Here we present a fast and reproducible method for 96-well filter plates and cells in suspension, a method that is best suited for agonist concentration-response studies and, thus, for ligand screening. The method offers tremendous time and effort savings and enables execution of large experimental series previously unattainable for AA release studies.
Place, publisher, year, edition, pages
2010. Vol. 407, no 2, 233-236 p.
Arachidonic acid, Phospholipase A(2), Lipid messengers, G-protein-coupled receptor signaling
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-133837DOI: 10.1016/j.ab.2010.07.014ISI: 000283034200012OAI: oai:DiVA.org:uu-133837DiVA: diva2:371015