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Filtration assay for arachidonic acid release
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Physiology.
2010 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 407, no 2, 233-236 p.Article in journal (Refereed) Published
Abstract [en]

Arachidonic acid (AA) release is a central message in cell signaling. Fatty acid release is generally assessed by manual sampling of radioactivity release from cells prelabeled with a radiolabeled fatty acid. The assay is laborious, time-consuming, and susceptible to high noise. Here we present a fast and reproducible method for 96-well filter plates and cells in suspension, a method that is best suited for agonist concentration-response studies and, thus, for ligand screening. The method offers tremendous time and effort savings and enables execution of large experimental series previously unattainable for AA release studies.

Place, publisher, year, edition, pages
2010. Vol. 407, no 2, 233-236 p.
Keyword [en]
Arachidonic acid, Phospholipase A(2), Lipid messengers, G-protein-coupled receptor signaling
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-133837DOI: 10.1016/j.ab.2010.07.014ISI: 000283034200012OAI: oai:DiVA.org:uu-133837DiVA: diva2:371015
Available from: 2010-11-18 Created: 2010-11-16 Last updated: 2010-11-18Bibliographically approved

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