uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
MicroRNA profiling of benign and malignant pheochromocytomas identifies novel diagnostic and therapeutic targets
Show others and affiliations
2010 (English)In: Endocrine-Related Cancer, ISSN 1351-0088, Vol. 17, no 3, 835-846 p.Article in journal (Refereed) Published
Abstract [en]

MicroRNAs (miRNAs) are small RNAs (similar to 22 bp) that post-transcriptionally regulate protein expression and are found to be differentially expressed in a number of human cancers. There is increasing evidence to suggest that miRNAs could be useful in cancer diagnosis, prognosis, and therapy. We performed miRNA microarray expression profiling on a cohort of 12 benign and 12 malignant pheochromocytomas and identified a number of differentially expressed miRNAs. These results were validated in a separate cohort of ten benign and ten malignant samples using real-time quantitative RT-PCR; benign samples had a minimum follow-up of at least 2 years. It was found that IGF2 as well as its intronic miR-483-5p was over-expressed, while miR-15a and miR-16 were under-expressed in malignant tumours compared with benign tumours. These miRNAs were found to be diagnostic and prognostic markers for malignant pheochromocytoma. The functional role of miR-15a and miR-16 was investigated in vitro in the rat PC12 pheochromocytoma cell line, and these miRNAs were found to regulate cell proliferation via their effect on cyclin D1 and apoptosis. These data indicate that miRNAs play a pivotal role in the biology of malignant pheochromocytoma, and represent an important class of diagnostic and prognostic biomarkers and therapeutic targets warranting further investigation.

Place, publisher, year, edition, pages
2010. Vol. 17, no 3, 835-846 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-134549DOI: 10.1677/ERC-10-0142ISI: 000282445100029PubMedID: 20621999OAI: oai:DiVA.org:uu-134549DiVA: diva2:372978
Available from: 2010-11-29 Created: 2010-11-29 Last updated: 2013-11-06Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Stålberg, Peter
By organisation
Endocrine Surgery
In the same journal
Endocrine-Related Cancer
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 157 hits
ReferencesLink to record
Permanent link

Direct link