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Conjugation of HPV16 E7 to cholera toxin enhances the HPV-specific T-cell recall responses to pulsed dendritic cells in vitro in women with cervical dysplasia
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
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2010 (English)In: Vaccine, ISSN 0264-410X, E-ISSN 1873-2518, Vol. 28, no 36, 5828-5836 p.Article in journal (Refereed) Published
Abstract [en]

We have evaluated whether cholera toxin (CT) as a carrier/adjuvant can enhance human T-cell responses to a viral oncoprotein in vitro using dendritic cells (DCs) as antigen-presenting cells. Monocyte-derived DCs obtained from women with cervical dysplasia were pulsed with the HPV16 oncoprotein E7, either alone or conjugated to CT, and tested for their ability to induce antigen-specific activation of autologous T cells in vitro. CT-conjugation of E7 significantly improved the capacity of pulsed DCs to activate antigen-specific CD4+ T-cell proliferation and IFN-gamma secretion. The CT-E7-pulsed DCs also produced significantly more of the Th1-inducing cytokine IL-12 compared to DCs pulsed with E7 or CT alone. Furthermore, DCs pulsed with CT-conjugated HPV16 E7 caused a response in T cells from women with advanced disease (CIN III) as well as in T cells from women that were currently not infected with HPV16. These data show the potential of using CT-conjugated viral oncoproteins for DC-induced T-cell activation in humans.

Place, publisher, year, edition, pages
2010. Vol. 28, no 36, 5828-5836 p.
Keyword [en]
Dendritic cells, Human papillomavirus, T cells
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-135026DOI: 10.1016/j.vaccine.2010.06.068ISI: 000281449000010OAI: oai:DiVA.org:uu-135026DiVA: diva2:374313
Available from: 2010-12-03 Created: 2010-12-03 Last updated: 2010-12-03Bibliographically approved

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