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MDMA (Ecstasy) decreases the number of neurons and stem cells in embryonic cortical cultures
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Functional Pharmacology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
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2010 (English)In: Cellular and molecular neurobiology, ISSN 0272-4340, E-ISSN 1573-6830, Vol. 30, no 1, 13-21 p.Article in journal (Refereed) Published
Abstract [en]

Ecstasy, 3,4-methylenedioxymetamphetamine (MDMA), is a recreational drug used among adolescents, including young pregnant women. MDMA passes the placental barrier and may therefore influence fetal development. The aim was to investigate the direct effect of MDMA on cortical cells using dissociated CNS cortex of rat embryos, E17. The primary culture was exposed to a single dose of MDMA and collected 5 days later. MDMA caused a dramatic, dose-dependent (100 and 400 microM) decrease in nestin-positive stem cell density, as well as a significant reduction (400 microM) in NeuN-positive cells. By qPCR, MDMA (200 microM) caused a significant decrease in mRNA expression of the 5HT3 receptor, dopamine D(1) receptor, and glutamate transporter EAAT2-1, as well as an increase in mRNA levels of the NMDA NR1 receptor subunit and the 5HT(1A) receptor. In conclusion, MDMA caused a marked reduction in stem cells and neurons in embryonic cortical primary cell cultures, which was accompanied by changes in mRNA expression of specific receptors and transporters for glutamatergic and monoaminergic neurotransmitters.

Place, publisher, year, edition, pages
2010. Vol. 30, no 1, 13-21 p.
Keyword [en]
5HT3 receptor, Cell culture, Cell death, Cortex, Dopamine D1 receptor, Embryos, Glutamate transporter, MDMA, MRNA, Neural stem cell
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-135071DOI: 10.1007/s10571-009-9426-yISI: 000274087400002PubMedID: 19543826OAI: oai:DiVA.org:uu-135071DiVA: diva2:374357
Available from: 2010-12-03 Created: 2010-12-03 Last updated: 2010-12-20Bibliographically approved

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