Prr1 Coat Protein Binding to its Translational Operator
2013 (English)In: Acta Crystallographica Section D: Biological Crystallography, ISSN 0907-4449, E-ISSN 1399-0047, Vol. 69, 367-372 p.Article in journal (Refereed) Published
In small RNA bacteriophages, the genomic RNA binds to the coat proteins when the viral capsid assembles. This is achieved through sequence-specific interactions between a coat-protein dimer and an RNA stem-loop that includes the start codon for the replicase gene. The structure of virus-like particles of the small RNA phage PRR1 bound to an RNAsegment corresponding to this stem-loop has been solved and the binding was compared with the related, and better investigated, phage MS2. The overall conformation of the RNA is found to be similar and the residues that are involved in RNA binding in PRR1 are the same as in MS2. The arrangement of the nucleotide bases in the loop of the stem-loop is different, leading to a difference in the stacking at the conserved Tyr86, which is equivalent to Tyr85 in MS2.
Place, publisher, year, edition, pages
2013. Vol. 69, 367-372 p.
PRR1, virus, coat protein, RNA
IdentifiersURN: urn:nbn:se:uu:diva-135156DOI: 10.1107/S0907444912047464ISI: 000316742700006OAI: oai:DiVA.org:uu-135156DiVA: diva2:374546
FunderSwedish Research Council