Serine- and Arginine-rich Proteins 55 and 75 (SRp55 and SRp75) Induce Production of HIV-1 vpr mRNA by Inhibiting the 5 '-Splice Site of Exon 3
2010 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, ISSN 20685659, Vol. 285, no 41, 31537-31547 p.Article in journal (Refereed) Published
HIV-1 non-coding exon 3 can either be spliced to exons 4, 4a, 4b, 4c, and 5 to generate tat, rev, and nef mRNAs or remain unspliced to produce the 13a7 vpr mRNA. Here we show that serine-and arginine-rich proteins 55 and 75 (SRp55 and SRp75) inhibit splicing from the 5'-splice site of exon 3 thereby causing an accumulation of the partially unspliced 13a7 vpr mRNA. In contrast, serine- and arginine-rich protein 40 (SRp40) induces splicing from exon 3 to exon 4, thereby promoting the production of the 1347 tat mRNA. We demonstrate that SRp55 stimulates vpr mRNA production by interacting with the previously identified HIV-1 splicing enhancer named GAR and inhibiting its function. This inhibition requires both serine arginine-rich and RNA-binding domains of SRp55, indicating that production of HIV-1 vpr mRNA depends on the interaction of SRp55 with an unknown factor.
Place, publisher, year, edition, pages
2010. Vol. 285, no 41, 31537-31547 p.
RNA/Splicing, Viruses/HIV, Gene Regulation, Human Immunodeficiency Virus, RNA Processing, SR Protein, SRp55, Alternative Splicing, vpr
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-135095DOI: 10.1074/jbc.M109.077453ISI: 000282764600048OAI: oai:DiVA.org:uu-135095DiVA: diva2:374722