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Response of H-2 production and Hox-hydrogenase activity to external factors in the unicellular cyanobacterium Synechocystis sp strain PCC 6803
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Photochemistry and Molecular Science, Microbial Chemistry.
2010 (English)In: International journal of hydrogen energy, ISSN 0360-3199, Vol. 35, no 13, 6611-6616 p.Article in journal (Refereed) Published
Abstract [en]

The effects of external factors on both H-2 production and bidirectional Hox-hydrogenase activity were examined in the non-N-2-fixing cyanobacterium Synechocystis PCC 6803. Exogenous glucose and increased osmolality both enhanced H-2 production with optimal production observed at 0.4% and 20 mosmol kg(-1), respectively. Anaerobic condition for 24 h induced significant higher H(2)ase activity with cells in BC11(0) showing highest activities. Increasing the pH resulted in an increased Hox-hydrogenase activity with an optimum at pH 7.5. The Hox-hydrogenase activity gradually increased with increasing temperature from 30 degrees C to 60 degrees C with the highest activity observed at 70 degrees C. A low concentration at 100 mu M of either DTT or beta-mercaptoethanol resulted in a minor stimulation of H-2 production. beta-Mercaptoethanol added to nitrogen- and sulfur-deprived cells stimulated H-2 production significantly. The highest Hox-hydrogenase activity was observed in cells in BG11(0)-S-deprived condition and 750 mu M beta-mercaptoethanol measured at a temperature of 70 degrees C; 14.32 mu mol H-2 mg chl alpha(-1) min(-1).

Place, publisher, year, edition, pages
2010. Vol. 35, no 13, 6611-6616 p.
Keyword [en]
Synechocystis PCC 6803, Hox-hydrogenase, H-2 production, H(2)ase activity, Nitrogen deprivation, beta-Mercaptoethanol
National Category
Chemical Sciences
URN: urn:nbn:se:uu:diva-135605DOI: 10.1016/j.ijhydene.2010.04.047ISI: 000280601600019OAI: oai:DiVA.org:uu-135605DiVA: diva2:375274
Available from: 2010-12-07 Created: 2010-12-07 Last updated: 2012-08-24Bibliographically approved

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