Response of H-2 production and Hox-hydrogenase activity to external factors in the unicellular cyanobacterium Synechocystis sp strain PCC 6803
2010 (English)In: International journal of hydrogen energy, ISSN 0360-3199, Vol. 35, no 13, 6611-6616 p.Article in journal (Refereed) Published
The effects of external factors on both H-2 production and bidirectional Hox-hydrogenase activity were examined in the non-N-2-fixing cyanobacterium Synechocystis PCC 6803. Exogenous glucose and increased osmolality both enhanced H-2 production with optimal production observed at 0.4% and 20 mosmol kg(-1), respectively. Anaerobic condition for 24 h induced significant higher H(2)ase activity with cells in BC11(0) showing highest activities. Increasing the pH resulted in an increased Hox-hydrogenase activity with an optimum at pH 7.5. The Hox-hydrogenase activity gradually increased with increasing temperature from 30 degrees C to 60 degrees C with the highest activity observed at 70 degrees C. A low concentration at 100 mu M of either DTT or beta-mercaptoethanol resulted in a minor stimulation of H-2 production. beta-Mercaptoethanol added to nitrogen- and sulfur-deprived cells stimulated H-2 production significantly. The highest Hox-hydrogenase activity was observed in cells in BG11(0)-S-deprived condition and 750 mu M beta-mercaptoethanol measured at a temperature of 70 degrees C; 14.32 mu mol H-2 mg chl alpha(-1) min(-1).
Place, publisher, year, edition, pages
2010. Vol. 35, no 13, 6611-6616 p.
Synechocystis PCC 6803, Hox-hydrogenase, H-2 production, H(2)ase activity, Nitrogen deprivation, beta-Mercaptoethanol
IdentifiersURN: urn:nbn:se:uu:diva-135605DOI: 10.1016/j.ijhydene.2010.04.047ISI: 000280601600019OAI: oai:DiVA.org:uu-135605DiVA: diva2:375274