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A prospective randomized sibling-oocyte study of two media systems for culturing cleavage-stage embryos-impact on fertilization rate
Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health. (Klinisk och experimentell reproduktionsbiologi/Olovsson)
2010 (English)In: Journal of Assisted Reproduction and Genetics, ISSN 1058-0468, E-ISSN 1573-7330, Vol. 28, no 4, 335-341 p.Article in journal (Refereed) Published
Abstract [en]

PURPOSE: Although several media systems have been developed, data from prospective randomised clinical studies are still lacking. In the present study we compared the effects of 2 different media systems on embryo morphology and development at days 2/3 using sibling oocytes. METHODS: In this prospective sibling-split trial, 1206 oocytes from 110 women were divided via alternate allocation to fertilization and culture in media system A (G-IVF (TM) v5 PLUS/ G-1(TM) v5 PLUS) or for fertilization and culture in media system B (Universal IVF medium/EmbryoAssist (TM)). RESULTS: The use of media system A significantly increased the normal fertilization rate (73.5% versus 67.2%; p = 0.030) and embryo utilization rate (55.5% versus 42.9%; p = 0.001), whereas polyploidy and embryo quality were similar in the two groups. CONCLUSION: The different impacts on fertilization and early embryo development between the two commercially available and commonly used media systems show the importance of evaluation of the efficacy of existing sequential culture media and the need to further improve media for in vitro development of human embryos.

Place, publisher, year, edition, pages
2010. Vol. 28, no 4, 335-341 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-135642DOI: 10.1007/s10815-010-9518-0ISI: 000291674100007PubMedID: 21120597OAI: oai:DiVA.org:uu-135642DiVA: diva2:375314
Available from: 2010-12-07 Created: 2010-12-07 Last updated: 2015-07-24Bibliographically approved

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