uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Diabetes Aggravates Nanoparticles Induced Breakdown of the Blood-Brain Barrier Permeability, Brain Edema Formation, Alterations in Cerebral Blood Flow and Neuronal Injury: An Experimental Study Using Physiological and Morphological Investigations in the Rat
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Anaesthesiology and Intensive Care.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Anaesthesiology and Intensive Care.
2010 (English)In: Journal of Nanoscience and Nanotechnology, ISSN 1533-4880, E-ISSN 1533-4899, Vol. 10, no 12, 7931-7945 p.Article in journal (Refereed) Published
Abstract [en]

The possibility that diabetes aggravates nanoparticles induced blood brain barrier (BBB) break-down, edema formation and brain pathology was examined in a rat model. Engineered nanoparticles from metals Ag and Cu (50-60 mn) were administered (50 mg/kg, i.p.) once daily for 7 days in normal and streptozotocine induced diabetic rats. On the 8th day, BBB permeability to Evans blue and radioactive iodine (I-131-sodium) was examined in 16 brain regions. In these brain regions alterations in regional CBF was also evaluated using radiolabelled (I-125) carbonized microspheres (o.d. 15 +/- 6 mu m). Regional brain edema and Na+, K+ and Cl- ion analysis were done in 8 selected brain regions. Histopathology was used to detect neuronal damage employing Nissl staining. Nanoparticles treatment in diabetic rats showed much more profound disruption of the BBB to Evans blue albumin (EBA) and radioiodine in almost all the 16 regions examined as compared to the normal animals. In these diabetic animals reduction in regional cerebral blood flow (CBF) was more pronounced than in normal rats. Edema development as seen using water content and increase in Na+ and a decrease in K+ ion were most marked in diabetic rats as compared to normal rats after nanoparticles treatment. Cell changes in the regions of BBB disruptions were also exacerbated in diabetic rats compared to normal group after nanoparticles treatment. Taken together, these observations are the first to show that diabetic rats are more susceptible to nanoparticles induced cerebrovascular reactions in the brain and neuronal damage. The possible mechanisms and significance of the present findings are discussed.

Place, publisher, year, edition, pages
2010. Vol. 10, no 12, 7931-7945 p.
Keyword [en]
Nanoparticles, Ag, Cu, Blood-Brain Barrier, Brain Edema, Cerebral Blood Flow, Neuronal Damage, Brain Pathology, Diabetes, Hyperglycemia
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-135339DOI: 10.1166/jnn.2010.3616ISI: 000283621400006OAI: oai:DiVA.org:uu-135339DiVA: diva2:375494
Available from: 2010-12-08 Created: 2010-12-06 Last updated: 2017-12-11Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Sharma, Hari Shanker

Search in DiVA

By author/editor
Sharma, Hari Shanker
By organisation
Anaesthesiology and Intensive Care
In the same journal
Journal of Nanoscience and Nanotechnology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 355 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf