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Proteome profiling of immortalization-to-senescence transition of human breast epithelial cells identified MAP2K3 as a senescence-promoting protein which is downregulated in human breast cancer
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm , Ludwig Institute for Cancer Research.
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2010 (English)In: PROTEOMICS - Clinical Applications, ISSN 1862-8346, Vol. 4, no 10-11, 816-828 p.Article in journal (Refereed) Published
Abstract [en]

Purpose: Immortalization is one of the first changes in cells undergoing carcinogenic transformation. Proteome profiling of the immortalization-senescence transition is expected to provide insights into the molecular mechanisms of early tumorigenesis. Experimental design: 2-DE and MALDI-MS were used to identify proteins in primary human breast epithelial cells, relevant to the immortalization-senescence transition. Cell and molecular biology and immunohistochemistry were used to validate involvement of mitogen-activated protein kinase kinase 3 (MAP2K3) in the immortalization-senescence transition. Results: We identified 71 proteins whose expression changed upon induction of senescence. The identified proteins include regulators of cell growth, death, cell assembly and organization. Analysis of the network formed by the identified proteins suggested that the immortalization-to-senescence transition could affect regulators of the cell cycle, protein synthesis, transport, post-translational modifications, DNA recombination and repair, and lipid and amino acid metabolism. We observed that MAP2K3 was downregulated in immortal human breast epithelial cells and that upregulation of MAP2K3 expression promoted cell senescence. Decreased expression of MAP2K3 was observed in human breast infiltrating ductal carcinomas, as compared to non-cancerous human breast tissues. Conclusion and clinical relevance: We described a proteome profile of the immortalization-to-senescence transition for human breast epithelial cells, and identified MAP2K3 as a protein that promotes senescence in these cells.

Place, publisher, year, edition, pages
2010. Vol. 4, no 10-11, 816-828 p.
Keyword [en]
Human breast epithelial cells, Immortalization, Mitogen-activated protein kinase kinase 3, Senescence
National Category
Cancer and Oncology
URN: urn:nbn:se:uu:diva-135308DOI: 10.1002/prca.201000006ISI: 000284044300004OAI: oai:DiVA.org:uu-135308DiVA: diva2:375702
Available from: 2010-12-08 Created: 2010-12-06 Last updated: 2010-12-08Bibliographically approved

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