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Biomarkers of oxidative stress in overweight men are not influenced by a combination of antioxidants
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences, Oxidative Stress and Inflammation. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences, Clinical Nutrition and Metabolism.
Department of Biosciences and Nutrition, Karolinska Institute.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences, Clinical Nutrition and Metabolism.
Department of Human Nutrition, Faculty of Life Science, University of Copenhagen.
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2010 (English)In: Free radical research, ISSN 1071-5762, E-ISSN 1029-2470, Vol. 44, no 5, 522-528 p.Article in journal (Refereed) Published
Abstract [en]

The effect of antioxidant supplementation on biomarkers of oxidative stress was investigated in a 6-week intervention study in 60 overweight men. The supplement contained a combination of antioxidants aiming to correspond to the antioxidant content found in a diet rich in fruit and vegetables. Placebo, single or double dose of antioxidants was provided to the subjects. Metabolic variables, plasma antioxidants and biomarkers of oxidative stress (lipid peroxidation and DNA damage) were measured. No effect of supplementation on biomarkers of oxidative stress was observed. Both intervention groups showed substantial increases of plasma antioxidants. This study demonstrated that supplementation with a combination of antioxidants did not affect lipid peroxidation and DNA damage in overweight men, despite increased concentrations of plasma antioxidants. The absence of antioxidant supplement effect might possibly be explained by the chosen study group having a normal level of oxidative stress, duration of the intervention and/or doses of antioxidants.

 

Place, publisher, year, edition, pages
2010. Vol. 44, no 5, 522-528 p.
Keyword [en]
Antioxidants, oxidative stress, isoprostanes, lipid peroxidation, DNA damage, overweight
National Category
Medical and Health Sciences
Research subject
Nutrition
Identifiers
URN: urn:nbn:se:uu:diva-135865DOI: 10.3109/10715761003647101ISI: 000276815300005OAI: oai:DiVA.org:uu-135865DiVA: diva2:375729
Available from: 2010-12-08 Created: 2010-12-08 Last updated: 2017-12-11Bibliographically approved
In thesis
1. Effect of Dietary Antioxidants on Oxidative Stress, Inflammation and Metabolic Factors: Studies in Subjects with Overweight and with Type 2 Diabetes
Open this publication in new window or tab >>Effect of Dietary Antioxidants on Oxidative Stress, Inflammation and Metabolic Factors: Studies in Subjects with Overweight and with Type 2 Diabetes
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Observational studies have indicated that fruit and vegetables, and dietary antioxidants may play an important role in reducing the risk of chronic diseases, potentially by affecting pathogenic mechanisms such as oxidative stress and inflammation. Clinical trials investigating the effects of supplementation with single or a few antioxidants in high doses have, however, shown inconsistent results and thus have not been able to support the observational findings.

It was therefore hypothesised that a supplement, containing a combination of antioxidants mainly extracted from fruit and vegetables, and supplied at moderate doses, might act more beneficially than single antioxidants given at pharmacological doses. The effects of such a supplement were investigated in two interventional studies described in this thesis. The effects on antioxidant status, metabolic control, oxidative stress and inflammation were investigated in overweight men and in patients with type 2 diabetes, subjects that could be expected to have elevated levels of oxidative stress and inflammatory activity.

The results of the studies did not support the hypothesis that supplementation with antioxidants from fruit and vegetables may have beneficial effects by counteracting oxidative stress and inflammation, despite markedly increased plasma antioxidant concentrations. However, interesting associations were observed in diabetes patients at baseline between intake of antioxidant rich food as well as levels of antioxidants in plasma, and markers of oxidative stress and inflammation. These associations are compatible with the hypothesis that a high intake of fruit and vegetables and dietary antioxidants decrease oxidative stress levels, have anti-inflammatory effects and a beneficial influence on glycaemic control. The results also indicated that glycaemic control may affect the level of oxidative stress.

The absence of beneficial effects from antioxidants might to some extent be explained by the initial levels of oxidative stress and inflammation and by the antioxidative status in the subjects included in the studies. Since the levels generally were comparable with those observed in healthy subjects, this might have decreased the ability to observe any beneficial effects of supplementation with additional antioxidants.

Continued investigations are needed to characterise the individuals who potentially might benefit from antioxidant supplementation. In view of apparent positive effects from a high intake of fruit and vegetables found in observational studies and until more knowledge is available from interventional trials about possible benefits and potential risks of antioxidant supplementation it still seems reasonable to recommend a diet rich in fruit and vegetables.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2011. 76 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 634
Keyword
Antioxidants, supplementation, fruit and vegetables, oxidative stress, isoprostanes, lipid peroxidation, oxidative damage to DNA, glycaemic control, inflammation, overweight, type 2 diabetes
National Category
Medical and Health Sciences
Research subject
Nutrition
Identifiers
urn:nbn:se:uu:diva-134938 (URN)978-91-554-7977-0 (ISBN)
Public defence
2011-02-04, Mariasalen, Stiftets Hus, Dragarbrunnsgatan 71, Uppsala, 13:15 (English)
Opponent
Supervisors
Available from: 2011-01-14 Created: 2010-12-02 Last updated: 2011-03-11Bibliographically approved

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