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Novel role for a bacterial nucleoid protein in translation of mRNAs with suboptimal ribosome-binding sites
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
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2010 (English)In: Genes & Development, ISSN 0890-9369, E-ISSN 1549-5477, Vol. 24, no 13, 1345-1350 p.Article in journal (Refereed) Published
Abstract [en]

In Escherichia coli, the major nucleoid protein H-NS limits transcription by acting as a repressor or transcriptional silencer, presumably by its ability to close the looped chromosome domains in the nucleoid through DNA-protein-DNA bridging. Here, we demonstrate the direct involvement of H-NS as a positive factor stimulating translation of the malT mRNA. In vitro studies showed that H-NS facilitates a repositioning of the 30S preinitiation complex on the malT mRNA. H-NS stimulation of translation depended on the AU-rich -35 to -40 region of the mRNA. Several additional examples were found demonstrating a novel function for H-NS in translation of genes with suboptimal ribosome-binding sequences.

Place, publisher, year, edition, pages
2010. Vol. 24, no 13, 1345-1350 p.
Keyword [en]
Bacterial nucleoid, gene expression, translation, ribosome-binding sites, 5 '-untranslated region
National Category
Biological Sciences
URN: urn:nbn:se:uu:diva-136029DOI: 10.1101/gad.576310ISI: 000279405000004OAI: oai:DiVA.org:uu-136029DiVA: diva2:376133
Available from: 2010-12-10 Created: 2010-12-09 Last updated: 2014-05-28Bibliographically approved

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Wagner, Gerhart E. H.
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Department of Cell and Molecular Biology
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