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Glucose-regulated protein 78 (Grp78/BiP) is secreted by human oviduct epithelial cells and the recombinant protein modulates sperm zona pellucida binding
Instituto de Biologia y Medicina Experimental, National Research Council of Argentina (CONICET), University of Buenos Aires, Buenos Aires, Argentina.
Centro Medico Fertilab, Buenos Aires, Argentina.
Laboratorio de Estudios Reproductivos, Area de Bioquimica Clinica, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Rosario, Argentina.
Laboratorio de Estudios Reproductivos, Area de Bioquimica Clinica, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Rosario, Argentina.
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2010 (English)In: Fertility and Sterility, ISSN 0015-0282, E-ISSN 1556-5653, Vol. 93, no 5, 1574-1584 p.Article in journal (Refereed) Published
Abstract [en]

Objective: To determine the secretion of Grp78 by human oviduct epithelial cells, its association to spermatozoa, and its involvement in gamete interaction. Design: Prospective study. Setting: Basic research laboratory. Subject(s): Semen samples obtained from normozoospermic volunteers. Tubal tissue provided by patients undergoing hysterectomies. Oocytes collected from women undergoing IVF-ET. Intervention(s): Analysis of Grp78 expression and secretion by oviductal tissue. Gamete incubation with recombinant Grp78 (rec-Grp78). Main Outcome Measure(s): Assessment of protein expression and secretion by immunohistochemistry and Western immunoblotting, respectively. Evaluation of rec-Grp78 binding to human spermatozoa by immunocytochemistry, and analysis of its effect upon gamete interaction using the hemizona assay. Result(s): Grp78 was found in the surface of oviduct epithelial cells. Soluble Grp78 was detected in oviductal fluids from women in the periovulatory period and in oviductal tissue conditioned medium. Rec-Grp78 was able to bind to the sperm acrosomal cap, and its presence during gamete interaction led to a decrease in the number of spermatozoa bound to the zona pellucida (ZP). When calcium ions from the incubation medium were replaced by strontium, rec-Grp78 enhanced sperm ZP interaction. Conclusion(S): Grp78 is expressed and secreted by oviduct epithelial cells. The protein would bind to the gametes and may modulate their interaction in a calcium-dependent manner.

Place, publisher, year, edition, pages
Elsevier Inc. , 2010. Vol. 93, no 5, 1574-1584 p.
Keyword [en]
Grp78, BiP, chaperone, oviduct, spermatozoa, zona pellucida, gamete interaction
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-136662DOI: 10.1016/j.fertnstert.2008.12.132ISI: 000276038700026PubMedID: 19296942OAI: oai:DiVA.org:uu-136662DiVA: diva2:377344
Available from: 2010-12-14 Created: 2010-12-14 Last updated: 2017-12-11Bibliographically approved

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